FastQCFastQC Report
Wed 25 May 2016
SRR1781088_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781088_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences119731
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGCCGT5235043.723012419507064No Hit
TCTTATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGCCG71785.99510569526689No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGCC26522.214965213687349No Hit
ATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGCCGTCTT13301.1108234291870944TruSeq Adapter, Index 7 (95% over 21bp)
TCTTTATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGCC13231.1049769900861097No Hit
CTATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGCCGTC9810.8193366797237139No Hit
ATTATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGCCGT8570.7157711870776992No Hit
CTTTATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGCCG5070.42344923202846374No Hit
CTTATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTAGTATGCCGT4860.4059099147255097No Hit
CTTTACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGCCGTC4720.3942170365235403No Hit
CTTATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGACGT4000.33408223434198325No Hit
CTTATACACATCTCCGAGCCCACGAGACCTTGGGTCATCACGTATGCCGT3910.32656538406928864No Hit
TCTTAATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGCC3590.29983880532192997No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3340.27895866567555605No Hit
TTATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGCCGTC3330.27812346008970107No Hit
CTTATACACATCTCCGAGCCCACGAGACCTAGGGTCATCTCGTATGCCGT3130.26141934837260195No Hit
CTTAATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGCCG3050.25473770368576226No Hit
CTTATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTAAGCCGT2920.24388003106964778No Hit
CTTATACACATCTCCGAGCCAACGAGACCTTGGGTCATCTCGTATGCCGT2860.23886879755451804No Hit
TATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGCCGTCT2800.2338575640393883No Hit
CATATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGCCGT2630.219659069079854No Hit
CTTATACACATCTCCGAGCCCACGAGACATTGGGTCATCTCGTATGCCGT2500.20880139646373957No Hit
CCTTATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGCCG2390.199614135019335No Hit
CTTATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGCAGT2080.1737227618578313No Hit
CTTATACACATCTCCGAGACCACGAGACCTTGGGTCATCTCGTATGCCGT1960.16370029482757179No Hit
TCTATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGCCGT1660.13864412725192307No Hit
CTTATACACATCTCAGAGCCCACGAGACCTTGGGTCATCTCGTATGCCGT1640.13697371608021314No Hit
CTTATACACATCTCCGAGCACACGAGACCTTGGGTCATCTCGTATGCCGT1380.11525837084798422No Hit
CTAATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGCCGT1320.11024713733285448No Hit
CTTATAAACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGCCGT1260.10523590381772473No Hit
CTTATACACATCTCCGAGCCCACGAGACCATGGGTCATCTCGTATGCCGT1230.10273028706015985No Hit
CTTATACACATCTCCGAGCCCACGAGACCTTGGGTCATATCGTATGCCGT1220.10189508147430491No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTACAC252.812656E-670.053512
ACGCATC150.002213302870.053516
TTATACG150.002213302870.053512
ATACGCA150.002213302870.053514
TATACGC150.002213302870.053513
CGTATAA207.9024816E-569.9949948
CAAGTAT207.9024816E-569.9949939
CGAAATA150.002220655669.9949948
TAAAGGG150.002220655669.9949967
CGCATCT150.002220655669.994997
AAGACGT252.8266659E-669.9949944
CGTCTAC207.9024816E-569.9949948
TTTCCAC150.002220655669.9949935
TGACGAA207.9024816E-569.9949945
ATCAAGT252.8266659E-669.9949937
GACTTCA150.002220655669.9949949
AGACGTC252.8266659E-669.9949945
ATATCGA150.002220655669.9949937
GCCGACT353.6779966E-969.9949946
AAACAGG150.002220655669.9949967
Produced by FastQC (version 0.11.5)