FastQCFastQC Report
Wed 25 May 2016
SRR1781087_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781087_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences611765
Sequences flagged as poor quality0
Sequence length76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG9440415.431415657973242No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCGC252.3903158E-455.9992143
CGACGGT200.00695087552.4992688
CTACGCT200.00695087552.4992684
ATGCGCG200.00695087552.4992684
CGCGTCT604.9349183E-946.666017
ACGCGTC305.877642E-446.666016
TGCGCGT651.0013537E-843.0763175
TGGTCGC1550.040.6445949
GCCGTAT1050.039.9994454
TCGCCGT1100.038.18128652
TCGGTGG2050.035.85315745
GTCGCCG1500.034.99950851
CGTATCA1400.034.99950856
CGGTGGT1950.034.10208546
ATCTCGG1500.032.66620642
CTCGGTG2100.031.66622244
TCGCGGT450.00430719831.11067452
GTATCAT1750.029.99957857
TCTCGGT2300.028.9126443
AATGCGT853.955447E-628.8231268
Produced by FastQC (version 0.11.5)