FastQCFastQC Report
Wed 25 May 2016
SRR1781087_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781087_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences611765
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTTATGAAAATCTCGTATGCCGT6191310.12038936519742No Hit
TCTTATACACATCTCCGAGCCCACGAGACTTATGAAAATCTCGTATGCCG88641.4489223803257787No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTTATGAAAATCTCGTATGCC32990.5392593561253095No Hit
ATACACATCTCCGAGCCCACGAGACTTATGAAAATCTCGTATGCCGTCTT19130.3127017727395323RNA PCR Primer, Index 19 (95% over 23bp)
TCTTTATACACATCTCCGAGCCCACGAGACTTATGAAAATCTCGTATGCC18380.30044216324896No Hit
CTATACACATCTCCGAGCCCACGAGACTTATGAAAATCTCGTATGCCGTC14150.23129796572213188No Hit
ATTATACACATCTCCGAGCCCACGAGACTTATGAAAATCTCGTATGCCGT13660.22328835418829124No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG78250.061.18707370
CGTCTAC700.054.9987248
AACTCGT1100.054.08964537
TCTACGA1850.052.97173711
ATCGTAA200.006951131452.49877539
AAACTCG1350.051.8506436
CGTCTTC95150.051.49643348
TCGTATG100600.051.38547540
GCCGTCT95300.051.37865446
TATGCCG97450.051.3584743
CTCGTAT99500.051.35558339
CGTATGC98950.051.322741
TCTCGTA99300.051.3180438
CCGTCTT94650.051.25078247
AATCTCG99750.051.19178836
ATCTCGT99750.051.08652537
GTATGCC98200.051.0018642
ATGCCGT97900.050.94365344
TGCCGTC97250.050.9242645
CTTGAAA99700.050.79620757