FastQCFastQC Report
Wed 25 May 2016
SRR1781086_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781086_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences919051
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG890879.693368485535624No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACCTT501.7102726E-434.9998114
TATGCGC604.983684E-429.166513
GCCGTAT657.9583685E-426.9229354
CGAGCAA700.001226406524.99986529
ATAGGCG851.30879E-424.7057484
CGTACAC750.001832810323.3332063
GTCTACG13600.021.10282570
TAGGCGC850.003790315220.5881235
TGGTCGC850.003790315220.58812349
GGTCTAC20050.020.07470570
AATCGCC900.005276242719.44433828
GGTATGC1301.0547422E-418.8522051
GGTATAA2601.4533725E-917.505621
GTGGTCG1200.001340840217.49990548
GAGGTCT36750.017.04752570
GCTCTAC1503.150555E-416.3385771
CGGTGGT1503.1584053E-416.33324446
TCTATAC3057.003109E-1016.0654873
AGGTCTA27800.015.86322370
ACACCGC1554.0542113E-415.8063658
Produced by FastQC (version 0.11.5)