FastQCFastQC Report
Wed 25 May 2016
SRR1781086_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781086_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences919051
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTTTAATGCATCTCGTATGCCGT567836.178438410926053No Hit
TCTTATACACATCTCCGAGCCCACGAGACTTTAATGCATCTCGTATGCCG60030.653173762935898No Hit
CATATACACATCTCCGAGCCCACGAGACTTTAATGCATCTCGTATGCCGT33250.3617862338433885No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTTTAATGCATCTCGTATGCC32970.35873961292681256No Hit
ATACACATCTCCGAGCCCACGAGACTTTAATGCATCTCGTATGCCGTCTT13950.1517870063794066Illumina PCR Primer Index 3 (95% over 21bp)
TCTTTATACACATCTCCGAGCCCACGAGACTTTAATGCATCTCGTATGCC12370.13459535977872827No Hit
CTATACACATCTCCGAGCCCACGAGACTTTAATGCATCTCGTATGCCGTC9870.10739338730930056No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG71350.064.600970
TATGCCG91650.054.11082543
CGTATGC92500.053.9919541
GCCGTCT90550.053.95649746
CCGTCTT90100.053.9540747
CGTCTTC90950.053.9500848
ATGCCGT92200.053.93987744
TCGTATG94350.053.9348340
CTCGTAT93700.053.86075639
TCTCGTA93800.053.84064538
TGCCGTC91900.053.7732145
ATCTCGT94100.053.7061937
GTATGCC92850.053.37380242
CATCTCG94700.053.1072236
TGCCGAC608.367351E-1152.4974345
GTCTTCT93800.052.23624449
CTTGAAA95500.052.0026957
CTGCTTG93900.051.7333654
GCATCTC97250.051.64271535
ATGCATC98050.051.3998333