FastQCFastQC Report
Wed 25 May 2016
SRR1781085_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781085_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences126956
Sequences flagged as poor quality0
Sequence length76
%GC84

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG8208464.6554711868679No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTACGC150.00222038569.9996143
GCGCGTC150.00222038569.999616
GATTTAC150.00222038569.9996141
TAGATTT150.00222038569.9996139
TTACGCC150.00222038569.9996144
CCAACCC150.00222038569.999613
CGCGTCT150.00222038569.999617
AAGTTAG207.901745E-569.999635
CTGGCGC207.901745E-569.999612
TCTGGCG252.8265476E-669.999611
CTCGGGG252.3829823E-455.99968344
AGTTAGA252.3829823E-455.99968336
GCGCTGG252.3829823E-455.99968315
ATTCCGG200.00692722152.520391
TCCGGAT200.00693802752.4997023
CGGCCGG200.00693802752.49970218
CCGGATA200.00693802752.4997024
GTCTCGG200.00693802752.49970242
GTTAGAT200.00693802752.49970237
CGCTGGC200.00693802752.49970216
Produced by FastQC (version 0.11.5)