FastQCFastQC Report
Wed 25 May 2016
SRR1781085_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781085_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences126956
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCTCATGGGATCTCGTATGCCGT5296341.717602949053216No Hit
TCTTATACACATCTCCGAGCCCACGAGACCTCATGGGATCTCGTATGCCG54534.295188884337882No Hit
CATATACACATCTCCGAGCCCACGAGACCTCATGGGATCTCGTATGCCGT28272.2267557263933964No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCTCATGGGATCTCGTATGCC24481.92822710230316No Hit
ATACACATCTCCGAGCCCACGAGACCTCATGGGATCTCGTATGCCGTCTT13391.0546961151895144RNA PCR Primer, Index 34 (95% over 23bp)
TCTTTATACACATCTCCGAGCCCACGAGACCTCATGGGATCTCGTATGCC11220.8837707552222818No Hit
CTATACACATCTCCGAGCCCACGAGACCTCATGGGATCTCGTATGCCGTC9010.7096946973754686No Hit
ACTTATACACATCTCCGAGCCCACGAGACCTCATGGGATCTCGTATGCCG5970.47024165852736377No Hit
CTTTATACACATCTCCGAGCCCACGAGACCTCATGGGATCTCGTATGCCG4420.34815211569362614No Hit
CTTTACACATCTCCGAGCCCACGAGACCTCATGGGATCTCGTATGCCGTC4220.33239862629572453RNA PCR Primer, Index 34 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACCTCATGGGATCTAGTATGCCGT3960.31191909007845237No Hit
CTTATACACATCTCCGAGCCCACGAGACCTCATGGGATATCGTATGCCGT3440.2709600176439081No Hit
CTTATACACATCTCCGAGCCCACGAGACCTCATGGGATCTCGTATGACGT3360.2646586218847475No Hit
CTTATACACATCTCCGAGCCAACGAGACCTCATGGGATCTCGTATGCCGT3120.2457544346072655No Hit
CTTAATACACATCTCCGAGCCCACGAGACCTCATGGGATCTCGTATGCCG3100.24417908566747534No Hit
CTTATACACATCTCCGAGCCCACGAGACCTCAAGGGATCTCGTATGCCGT3020.23787768990831468No Hit
TTATACACATCTCCGAGCCCACGAGACCTCATGGGATCTCGTATGCCGTC2960.23315164308894418No Hit
CTAATACACATCTCCGAGCCCACGAGACCTCATGGGATCTCGTATGCCGT2900.2284255962695737No Hit
TCTTAATACACATCTCCGAGCCCACGAGACCTCATGGGATCTCGTATGCC2700.2126721068716721No Hit
TATACACATCTCCGAGCCCACGAGACCTCATGGGATCTCGTATGCCGTCT2600.20479536217272126RNA PCR Primer, Index 34 (95% over 22bp)
CTTATACACATCTCCGAGCCCACGAGACCTCATGGATCTCGTATGCCGTC2530.1992816408834557No Hit
CTTATACACATCTCCGAGCCCACGAGACCTCATGGGATCTCGTAAGCCGT2240.17643908125649832No Hit
CCTTATACACATCTCCGAGCCCACGAGACCTCATGGGATCTCGTATGCCG2200.173288383376918No Hit
CTTATACACATCTCCGAGCCCACGAGACATCATGGGATCTCGTATGCCGT2170.17092535996723274No Hit
CTTATACACATCTCCGAGCCCACGAGACCTCATGGGATCTCGTATGCAGT2160.17013768549733768No Hit
CTTAAACACATCTCCGAGCCCACGAGACCTCATGGGATCTCGTATGCCGT1420.1118497747251016No Hit
TCTATACACATCTCCGAGCCCACGAGACCTCATGGGATCTCGTATGCCGT1280.10082233214657046No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCTCTT150.002220335670.0000147
CAGTTCA150.002220335670.0000144
ATATGCA150.002220335670.0000152
AGGAACC150.002220335670.0000125
CCAGATG150.002220335670.0000130
TATGACC150.002220335670.0000111
CGGGGGG150.002220335670.0000170
TCGGATA150.002220335670.0000137
AATGACG150.002220335670.0000143
CGGATAC150.002220335670.0000138
ACCAGAT150.002220335670.0000129
GGAACCA150.002220335670.0000126
ATCTTGA150.002220335670.0000155
ACTGTGA150.002220335670.0000142
TCACTTT150.002220335670.0000148
CTCGAAA150.002220335670.0000139
GGATTAC150.002220335670.000011
GACGACT150.002220335670.0000146
CCTATGC150.002220335670.0000141
ACTTTAG150.002220335670.0000150
Produced by FastQC (version 0.11.5)