FastQCFastQC Report
Wed 25 May 2016
SRR1781084_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781084_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences227520
Sequences flagged as poor quality0
Sequence length76
%GC61

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG5758525.309862869198312No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGGCG353.6961865E-969.9986811
TGCGCGT352.9656803E-759.9988675
CGCGTCT308.359531E-658.332237
GAATAGA200.006921302552.5452081
TCGCCAA200.006945424252.49901614
AATGCGA200.006945424252.4990166
TGATTCG200.006945424252.49901643
TTCGGGA200.006945424252.49901646
TAATGCG200.006945424252.4990165
CGCCCAT200.006945424252.49901652
ATGCGCG305.869957E-446.6657874
GCGCGTC404.583646E-543.749186
TGCCGGC404.583646E-543.7491819
CTGCCGG400.002415593234.99934418
GCAGGTG400.002415593234.99934456
TAGTATC400.002415593234.99934431
TACTTAT652.0969266E-532.30708364
CCGGCGA450.00430166831.11052521
TGGCGCT853.941248E-628.82298713
CTATATG500.007199070427.99947442
Produced by FastQC (version 0.11.5)