FastQCFastQC Report
Wed 25 May 2016
SRR1781084_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781084_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences227520
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACACCGGCTAATCTCGTATGCCGT3445115.141965541490856No Hit
TCTTATACACATCTCCGAGCCCACGAGACACCGGCTAATCTCGTATGCCG39191.7224859353023911No Hit
CATATACACATCTCCGAGCCCACGAGACACCGGCTAATCTCGTATGCCGT22590.992879746835443No Hit
CTCTTATACACATCTCCGAGCCCACGAGACACCGGCTAATCTCGTATGCC19270.8469585091420534No Hit
ATACACATCTCCGAGCCCACGAGACACCGGCTAATCTCGTATGCCGTCTT9930.4364451476793249TruSeq Adapter, Index 10 (95% over 21bp)
TCTTTATACACATCTCCGAGCCCACGAGACACCGGCTAATCTCGTATGCC8190.3599683544303798No Hit
CTATACACATCTCCGAGCCCACGAGACACCGGCTAATCTCGTATGCCGTC6890.30283052039381153No Hit
ACTTATACACATCTCCGAGCCCACGAGACACCGGCTAATCTCGTATGCCG5630.2474507735583685No Hit
CTTTACACATCTCCGAGCCCACGAGACACCGGCTAATCTCGTATGCCGTC3280.1441631504922644No Hit
CTTTATACACATCTCCGAGCCCACGAGACACCGGCTAATCTCGTATGCCG2800.12306610407876231No Hit
CTTATACACATCTCCGAGCCCACGAGACACAGGCTAATCTCGTATGCCGT2760.12130801687763715No Hit
TATACACATCTCCGAGCCCACGAGACACCGGCTAATCTCGTATGCCGTCT2650.1164732770745429No Hit
CTAATACACATCTCCGAGCCCACGAGACACCGGCTAATCTCGTATGCCGT2520.11075949367088608No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGAAAG207.9157144E-569.9986940
CGCATTA150.002222771569.9986845
TGACGTA252.8339873E-669.9986845
AACGGAT150.002222771569.9986829
ACGGATA150.002222771569.9986830
CGTCATA150.002222771569.9986848
AGGGGGG43000.063.8941570
ACGTATG501.4551915E-1162.99881440
AAGCCGT800.061.24884844
AGCCGTC650.059.22965245
GACCACG1250.058.79889318
AACTCGT800.056.8739337
AAACTCG1000.055.99894736
AAATCCG252.3871708E-455.99894710
ACCGGCA1250.055.99894729
AATCACG1000.055.99894736
CCGGCAA1250.055.99894730
GATCCCA252.3871708E-455.99894716
CGGATAA509.767973E-1055.99894731
CTCGAAA252.3871708E-455.99894739
Produced by FastQC (version 0.11.5)