FastQCFastQC Report
Wed 25 May 2016
SRR1781083_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781083_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences72585
Sequences flagged as poor quality0
Sequence length76
%GC85

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4909567.63794172349658No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGCCGG150.002216489370.018
CATTTCT200.006925944252.529
CTGGCGC200.006925944252.512
ATAGTTA200.006925944252.555
GGCGGAG200.006925944252.555
TCGGGGG450.00428216231.11111345
GGTGGCG500.00716662527.99999817
GACGAGG952.7467735E-422.10526370
CGACGAG1107.385215E-419.09090869
CCGACGA1159.956809E-418.2608768
GCCGACG1602.5533142E-517.570
GCTGCCG2201.3514E-615.9090967
TGCCGAC1807.004453E-515.55555669
CTGCCGA1807.004453E-515.55555668
CGCTGCC2556.262224E-613.72549166
ACGCTGC2559.5709474E-512.352941565
TGACGCT2851.9695788E-512.28070263
GACGCTG2952.804729E-511.86440764
CTGACGC3053.943823E-511.475409562
TCTGACG3351.02432175E-410.44776161
Produced by FastQC (version 0.11.5)