FastQCFastQC Report
Wed 25 May 2016
SRR1781083_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781083_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences72585
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGCCGT2902039.98071226837501No Hit
TCTTATACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGCCG33974.680030309292554No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGCC16112.2194668319900805No Hit
CATATACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGCCGT15052.0734311496865745No Hit
ATACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGCCGTCTT9871.359785079561893RNA PCR Primer, Index 14 (95% over 21bp)
TCTTTATACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGCC7431.0236274712406144No Hit
CTATACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGCCGTC6380.8789694840531791No Hit
ACTTATACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGCCG4140.5703657780533168No Hit
CTTTACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGCCGTC3540.487704071089068No Hit
CTTTATACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGCCG2550.3513122545980575No Hit
CTTATACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGACGT2380.3278914376248536No Hit
TCTTAATACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGCC2310.31824757181235797No Hit
TTATACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGCCGTC2270.31273679134807464No Hit
CTTATACACATCTCCGAGCCCACGAGACCGGTTCACATCTCGTATGCCGT2220.30584831576772065No Hit
TATACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGCCGTCT2210.30447062065164976No Hit
CTTATACACATCTCCGAGCCCACGAGACCGGTTACCATCTCGTATGCCGT1980.2727836329820211No Hit
CTTATACACATCTCCGAGCCCACGAGACAGGTTCCCATCTCGTATGCCGT1980.2727836329820211No Hit
CTTATACACATCTCCGAGCCCACGAGACCGGTTCCAATCTCGTATGCCGT1910.26313976716952536No Hit
CTTAATACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGCCG1900.2617620720534546No Hit
CTTATACACATCTCCGAGCCCACGAGACCGGATCCCATCTCGTATGCCGT1840.2534959013570297No Hit
CTTATACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGCAGT1770.243852035544534No Hit
CTTATACACATCTCCGAGCCAACGAGACCGGTTCCCATCTCGTATGCCGT1360.18736653578563064No Hit
CTAATACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGCCGT1330.1832334504374182No Hit
TCTATACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGCCGT1150.15843493834814354No Hit
CCTTATACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGCCG1140.15705724323207276No Hit
CTTATACACATCTCCGAGCCCACGAGACCGGTTCCCATATCGTATGCCGT1030.14190259695529378No Hit
ACTTTATACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGCC970.1336364262588689No Hit
CTTAAACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGCCGT940.12950334091065646No Hit
CTTATACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGCCGA850.11710408486601916No Hit
CTCTATACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGCCG760.10470482882138184No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTGCTT150.002217008569.99586553
ATCCCAT700.069.99586532
AGCCCAA353.6507117E-969.99586517
ACTCGTA500.069.99586538
ACTCGAA150.002217008569.99586538
ATCCAAT207.881042E-569.99586532
AAATCGA150.002217008569.99586537
ACGTCAT150.002217008569.99586547
CAGTATA150.002217008569.99586547
TAAGCCG252.8152244E-669.99586543
CAACTCG550.069.99586536
GAATGAC150.002217008569.99586542
AGAACGC150.002217008569.99586525
AACTCGT500.069.99586537
AACTCGA150.002217008569.99586537
GGTTACA252.8152244E-669.99586530
GGTTAAC150.002217008569.99586530
TACAATC150.002217008569.99586533
CCCAACT401.3278623E-1069.99586534
GCAGTAT207.881042E-569.99586546
Produced by FastQC (version 0.11.5)