FastQCFastQC Report
Wed 25 May 2016
SRR1781082_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781082_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences136996
Sequences flagged as poor quality0
Sequence length76
%GC74

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG5967343.5582060790096No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCCGT200.006939321352.4994535
TTGGACA200.006939321352.4994536
CCTACAA200.006939321352.4994522
TATGCTT350.001251964539.999585
GAGAGCA500.007188924627.9997117
ACAGCGC500.007188924627.999718
AACTCAA500.007188924627.9997138
ACTCAAA500.007188924627.9997139
GGTCTAC1709.172072E-820.5880270
AGGTCTA1751.2453893E-719.9997969
ACGAGGT3250.018.30750370
CAGCTGT1250.001752040716.79982444
GAGGTCT2108.4485873E-716.66649268
GTGTGGG1300.002277141216.1536792
GGTGTGG1554.0007022E-415.8178371
GACGAGG4300.015.46495669
CCGACGA6150.014.79659470
CGAGGTC2454.2044576E-614.28556567
CGACGAG4801.8189894E-1213.85402268
GCCGACG7350.012.38082469
Produced by FastQC (version 0.11.5)