FastQCFastQC Report
Wed 25 May 2016
SRR1781082_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781082_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences136996
Sequences flagged as poor quality0
Sequence length76
%GC48

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTCGTATGCCGT136499.963064615025257No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTAGTATGCCGT33442.4409471809395895No Hit
TCTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTCGTATGCCG10210.7452772343718065No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCGCGTATGCCGT10180.7430873894128296No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTCGTATTCCGT8890.6489240561768227No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCGAGTATGCCGT6100.44526847499197053No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTAGTATTCCGT5660.41315074892697595No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTCGTATGCC4920.3591345732722123No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTCGTATGCCCT4800.35037519343630474No Hit
TCTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTAGTATGCCG4460.3255569505678998No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTCGTATGACGT4050.2956290694618821No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTCGTATGCCGG3990.2912493795439283No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTCGTAAGCCGT3510.25621186020029785No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTCGGATGCCGT3290.24015299716780053No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTAGTATGACGT3070.22409413413530324No Hit
CATATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTCGTATGCCGT2950.2153347542993956No Hit
TCTTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTCGTATGCC2370.17299775175917545No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTAGTATGCCCT2320.1693480101608806No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTCGTATGGCGT2230.1627784752839499No Hit
CTTATACACATCTCCGAGCCCACGAGACATCGCGGCATCTCGTATGCCGT1990.14525971561213466No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCGCGTATGCCGG1960.14306987065315777No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCACGTATGCCGT1910.1394201290548629No Hit
TCTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCGCGTATGCCG1750.12774095594031942No Hit
CTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTCGTATGCCGTC1720.12555111098134253No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCGCGGATGCCGT1690.12336126602236562No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTAGTATGCCGG1610.11752167946509388No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTCGTATGCCGA1580.11533183450611698No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTCGTTTGCCGT1540.11241204122748111No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCGCGTATTCCGT1530.11168209290782212No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTAGTAAGCCGT1480.10803235130952729No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCAAGTATGCCGT1450.10584250635055038No Hit
TCTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTAGTATTCCG1390.10146281643259657No Hit
TCTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCGAGTATGCCG1380.1007328681129376No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACAACGC150.002220710570.0000127
CGGGTTA150.002220710570.0000148
CGTGGAA150.002220710570.0000157
GATAAGC150.002220710570.0000151
TACTTGG150.002220710570.000017
TCCGATC150.002220710570.0000113
TCCGACA150.002220710570.0000146
CTATAAC150.002220710570.0000142
ATCACGG150.002220710570.0000137
ATCCGTA301.01756086E-770.0000145
CCGTATA150.002220710570.0000147
CACTAGA150.002220710570.0000121
TCGTCTG150.002220710570.0000150
GGTATAG150.002220710570.000012
ATAAGCG150.002220710570.0000152
ACGTAAA150.002220710570.0000147
GCGTGGA150.002220710570.0000156
CGACTAT150.002220710570.0000139
GCGTAAG150.002220710570.0000140
CGTATAA207.9037454E-570.048
Produced by FastQC (version 0.11.5)