FastQCFastQC Report
Wed 25 May 2016
SRR1781081_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781081_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences224228
Sequences flagged as poor quality0
Sequence length76
%GC63

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG6710329.92623579570794No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTCT301.0205986E-769.999337
CTATAGA200.006926676752.534651
CCGTCAT200.006945038752.499514
TGCGAGT200.006945038752.49959
CCTTTCG200.006945038752.49959
GGGCCGT200.006945038752.499514
GCCGTTT200.006945038752.499522
GCGCGTC352.08217E-549.999526
TGGCGGC305.8695045E-446.66621819
TGCGCGT305.8695045E-446.6662185
GACTTCC404.5831814E-543.74958423
GCCTACA350.001249546940.0263941
CGTCTCA350.001253667439.99961525
TTCCCTA459.1787355E-538.88851526
CGTCTGG654.84717E-737.6919489
GTCGTGT400.002415408834.9996712
GCGTCTG601.2122713E-534.9996648
TCTGGCG601.2122713E-534.99966411
ATTTACG450.004301340331.11081342
ACTCAAT450.004301340331.11081340
Produced by FastQC (version 0.11.5)