FastQCFastQC Report
Wed 25 May 2016
SRR1781081_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781081_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences224228
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGACGCTCCATCTCGTATGCCGT4122318.38441229462868No Hit
TCTTATACACATCTCCGAGCCCACGAGACGACGCTCCATCTCGTATGCCG46582.0773498403410815No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGACGCTCCATCTCGTATGCC25991.1590880710705176No Hit
CATATACACATCTCCGAGCCCACGAGACGACGCTCCATCTCGTATGCCGT24861.1086929375457124No Hit
CTTATACACATCTCCGAGCCCACGAGACGCCGCTCCATCTCGTATGCCGT13180.587794566245072No Hit
ATACACATCTCCGAGCCCACGAGACGACGCTCCATCTCGTATGCCGTCTT10710.47763883190324136Illumina PCR Primer Index 11 (95% over 22bp)
TCTTTATACACATCTCCGAGCCCACGAGACGACGCTCCATCTCGTATGCC7860.3505360615088214No Hit
CTATACACATCTCCGAGCCCACGAGACGACGCTCCATCTCGTATGCCGTC7200.3211017357332715No Hit
ACTTATACACATCTCCGAGCCCACGAGACGACGCTCCATCTCGTATGCCG5620.2506377437251369No Hit
CTTTACACATCTCCGAGCCCACGAGACGACGCTCCATCTCGTATGCCGTC3680.16411866493033875No Hit
CTTTATACACATCTCCGAGCCCACGAGACGACGCTCCATCTCGTATGCCG3420.15252332447330397No Hit
CTTATACACATCTCCGAGCCAACGAGACGACGCTCCATCTCGTATGCCGT2750.12264302406479119No Hit
TTATACACATCTCCGAGCCCACGAGACGACGCTCCATCTCGTATGCCGTC2730.12175107479886545No Hit
CTTATACACATCTCCGAGCCCACGAGACGACGCTCCATCTCGTATGACGT2580.1150614553044223No Hit
CTTAATACACATCTCCGAGCCCACGAGACGACGCTCCATCTCGTATGCCG2440.10881781044294199No Hit
CTTATACACATCTCCGAGCCCACGAGACGACGCTCCATCTCGTATGCAGT2380.10614196264516473No Hit
TCTTAATACACATCTCCGAGCCCACGAGACGACGCTCCATCTCGTATGCC2350.10480403874627611No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACCGA207.9147256E-570.0000111
ACGTATT207.9147256E-570.0000147
ATAACCG207.9147256E-570.0000110
GACGACT252.8335362E-670.0000146
CGATCCA252.8335362E-670.0000131
GCCGTAA150.002222562770.0000146
AGGGGGG51950.065.351370
GACGATC453.8380676E-1062.22222529
TGCCGTA453.8380676E-1062.22222545
CGACGAT401.061926E-861.25000428
GCCGTAT352.9650982E-760.00000446
CATATAC6600.058.863641
TGACGAC308.3582E-658.33333645
CGCTACA601.8189894E-1258.33333631
ATGACGA308.3582E-658.33333644
CGTATGA1500.056.00000441
ACGACTT252.3868735E-456.00000447
ACGACGA509.767973E-1056.00000427
ACGAAAG252.3868735E-456.00000440
ACGTATG950.055.2631640
Produced by FastQC (version 0.11.5)