FastQCFastQC Report
Wed 25 May 2016
SRR1781080_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781080_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42838
Sequences flagged as poor quality0
Sequence length76
%GC65

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1335931.184929268406552No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGCTG150.002210258370.066
AAACCTA200.006906623552.529
GGCTGCT200.006906623552.532
CCCTGAA200.006906623552.561
GATAGCT200.006906623552.565
GGGCTGC200.006906623552.531
GAGGAGA500.007134283427.9999989
CGACGAG850.003732332720.58823670
CCGACGA1000.00954325717.569
Produced by FastQC (version 0.11.5)