FastQCFastQC Report
Wed 25 May 2016
SRR1781080_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781080_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42838
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGGAGTAAGATCTCGTATGCCGT602014.052943648162847No Hit
TCTTATACACATCTCCGAGCCCACGAGACGGAGTAAGATCTCGTATGCCG8021.8721695690741864No Hit
ATACACATCTCCGAGCCCACGAGACGGAGTAAGATCTCGTATGCCGTCTT7551.7624538960735796TruSeq Adapter, Index 9 (95% over 21bp)
CATATACACATCTCCGAGCCCACGAGACGGAGTAAGATCTCGTATGCCGT4861.1345067463467016No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGGAGTAAGATCTCGTATGCC3330.7773472150894066No Hit
CTATACACATCTCCGAGCCCACGAGACGGAGTAAGATCTCGTATGCCGTC2490.5812596293010879No Hit
TCTTTATACACATCTCCGAGCCCACGAGACGGAGTAAGATCTCGTATGCC2410.5625846211307717No Hit
CTTTACACATCTCCGAGCCCACGAGACGGAGTAAGATCTCGTATGCCGTC1870.4365283159811382No Hit
ACTTATACACATCTCCGAGCCCACGAGACGGAGTAAGATCTCGTATGCCG1750.40851580372566415No Hit
CTTATACACATCTCCGAGCCCACGAGACGGAGAAAGATCTCGTATGCCGT1170.2731219944908726No Hit
TATACACATCTCCGAGCCCACGAGACGGAGTAAGATCTCGTATGCCGTCT1140.26611886642700405No Hit
CTTATACACATCTCCGAGCCCACGAGACGCGTTAAAATCTCGTATGCCGT1000.23343760212895093No Hit
CTTAATACACATCTCCGAGCCCACGAGACGGAGTAAGATCTCGTATGCCG990.2311032261076614No Hit
CTTTATACACATCTCCGAGCCCACGAGACGGAGTAAGATCTCGTATGCCG950.2217657220225034No Hit
TCTTAATACACATCTCCGAGCCCACGAGACGGAGTAAGATCTCGTATGCC850.19842196180960828No Hit
TTATACACATCTCCGAGCCCACGAGACGGAGTAAGATCTCGTATGCCGTC800.18675008170316076No Hit
CTTATACACATCTCCGAGACCACGAGACGGAGTAAGATCTCGTATGCCGT580.13539380923479155No Hit
CTTATACACATCTCCGAGCCCACGAGACGGAGTAAGATCTCGTATGACGT530.123721929128344No Hit
CTTATACACATCTCCGAGCCAACGAGACGGAGTAAGATCTCGTATGCCGT520.12138755310705449No Hit
CTAATACACATCTCCGAGCCCACGAGACGGAGTAAGATCTCGTATGCCGT480.11205004902189646No Hit
CTTATACACATCTCCGAGCCCACGAGACGGAGTAAATCTCGTATGCCGTC450.10504692095802792No Hit
CTTATACACATCACCGAGCCCACGAGACGGAGTAAGATCTCGTATGCCGT430.10037816891544889No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTTACA301.0008989E-770.01
GTATCCC150.002210258370.042
AGACGAA150.002210258370.025
CGTATCC150.002210258370.041
TCGTATC150.002210258370.040
ATCCGAG150.002210258370.012
GAGACGA150.002210258370.024
ACACCGA401.035005E-861.2511
ACGGAGA800.061.2527
AACACCG401.035005E-861.2510
CACCGAG950.058.94736512
CGGAGAA850.057.6470628
ATCACCG553.45608E-1157.2727310
TCACCGA553.45608E-1157.2727311
AGGGGGG11250.056.31110870
ACCACGA750.056.019
AGACCAC750.056.017
GACCACG750.056.018
ACTCGTA252.3650329E-455.99999638
AGAACTC252.3650329E-455.99999635
Produced by FastQC (version 0.11.5)