FastQCFastQC Report
Wed 25 May 2016
SRR1781079_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781079_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences206880
Sequences flagged as poor quality0
Sequence length76
%GC67

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG7480436.15815931941222No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGCGGT200.006944304352.4994622
GCGCGTC200.006944304352.499466
TTAGCGA200.006944304352.4994627
CACCTCG200.006944304352.4994670
TACGAAC200.006944304352.4994628
CTTTCGC200.006944304352.4994629
GCTAAAC200.006944304352.4994613
AGGGATC200.006944304352.4994636
TGCACAG200.006944304352.499465
ACGAACG200.006944304352.4994629
AGAGTTC305.868467E-446.6661836
CCTTTTA404.581966E-543.7495558
CTTATTG350.001253447139.99958433
TGCCGGC350.001253447139.99958419
TGTTCCA400.002414987434.9996385
GCCGGCG552.9852169E-431.81785220
AGATCTC450.004300595331.11078840
CTGCCGG450.004300595331.11078818
CAATCTC450.004300595331.11078826
CGGTGGT604.9706444E-429.16636546
Produced by FastQC (version 0.11.5)