FastQCFastQC Report
Wed 25 May 2016
SRR1781079_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781079_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences206880
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCATCCTGTATCTCGTATGCCGT4462021.568058778035574No Hit
TCTTATACACATCTCCGAGCCCACGAGACCATCCTGTATCTCGTATGCCG46472.24622969837587No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCATCCTGTATCTCGTATGCC25141.2151972157772621No Hit
CATATACACATCTCCGAGCCCACGAGACCATCCTGTATCTCGTATGCCGT18070.8734532095901005No Hit
CTTATACACATCTCCGAGCCCACGAGACCATCCTGTATCTAGTATGCCGT16550.7999806651198763No Hit
ATACACATCTCCGAGCCCACGAGACCATCCTGTATCTCGTATGCCGTCTT9040.43696829079659705TruSeq Adapter, Index 2 (95% over 21bp)
TCTTTATACACATCTCCGAGCCCACGAGACCATCCTGTATCTCGTATGCC8880.42923433874709976No Hit
CTATACACATCTCCGAGCCCACGAGACCATCCTGTATCTCGTATGCCGTC6220.30065738592420727No Hit
CTTATACACATCTCCGAGCCCACGAGACAATCCTGTATCTCGTATGCCGT5310.2566705336426914No Hit
CTTATACACATCTCCGAGCCCACGAGACCATCCTGTATCTCGTATGACGT4660.22525135344160868No Hit
CTTATACACATCTCCGAGCCCACGAGACCATCCTGTATCTCGTAAGCCGT4020.1943155452436195No Hit
ACTTATACACATCTCCGAGCCCACGAGACCATCCTGTATCTCGTATGCCG3810.1841647331786543No Hit
CTTTACACATCTCCGAGCCCACGAGACCATCCTGTATCTCGTATGCCGTC3170.15322892498066512No Hit
CTTTATACACATCTCCGAGCCCACGAGACCATCCTGTATCTCGTATGCCG2890.13969450889404486No Hit
CATATACACATCTCCGAGCCCACGAGACCATCCTGTATCTAGTATGCCGT2670.1290603248259861No Hit
TCTTATACACATCTCCGAGCCCACGAGACCATCCTGTATCTAGTATGCCG2650.1280935808197989No Hit
TTATACACATCTCCGAGCCCACGAGACCATCCTGTATCTCGTATGCCGTC2490.12035962877030162No Hit
TCTTAATACACATCTCCGAGCCCACGAGACCATCCTGTATCTCGTATGCC2310.11165893271461717No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACCGA150.002220614670.0135411
AATGACG207.9151745E-569.9966243
ATACCGT150.002222745469.9966144
GTACGGA150.002222745469.9966115
AGGGGGG52450.066.1264570
AACTCGT550.063.63328637
GACGTAT352.9649527E-759.99709746
CATATAC6400.059.6064951
TAACTCG650.059.22790536
GCCGTCA6050.058.4269346
AACACGT308.35832E-658.33051337
CCGTCAT6250.057.1172447
GCCGTAA1050.056.6639346
TTCCGTA252.3870102E-455.9972945
CGAGAAC509.767973E-1055.9972923
CGTCATA1950.055.63833648
CGTCATC4950.055.14884648
GCATGAA7300.055.1343256
CGTCTTC59250.054.99312248
TGCCGTC70050.054.95808845
Produced by FastQC (version 0.11.5)