FastQCFastQC Report
Wed 25 May 2016
SRR1781078_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781078_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences227684
Sequences flagged as poor quality0
Sequence length76
%GC67

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG7490532.89866657296955No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTTCG150.0022226969.99934423
CGTTCGG150.0022226969.99934424
CGCCATT150.0022226969.99934433
CTACACG200.006927088452.534121
TCGCAAT200.00694517352.49950431
CGTGATA200.00694517352.49950425
AAGACCG200.00694517352.49950414
TTCCGCG200.00694517352.49950424
TATGCTC200.00694517352.4995045
CGAGCAA200.00694517352.49950431
TTCGGTA200.00694517352.49950426
CGCAATG200.00694517352.49950432
CGGTAAG200.00694517352.49950428
GACTATA350.001249648940.0259931
CTAATCG350.001253707139.99962227
TACGAGT400.002415485734.999674
ACATCGC400.002415485734.9996724
GGCTAAA450.004287617731.1313321
CGTTTAC450.00430147531.11081747
AACCGTG703.478912E-529.9997188
Produced by FastQC (version 0.11.5)