FastQCFastQC Report
Wed 25 May 2016
SRR1781078_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781078_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences227684
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGCGTTAAAATCTCGTATGCCGT4778220.986103547021308No Hit
TCTTATACACATCTCCGAGCCCACGAGACGCGTTAAAATCTCGTATGCCG49612.17889706786599No Hit
CATATACACATCTCCGAGCCCACGAGACGCGTTAAAATCTCGTATGCCGT29971.3162980270901776RNA PCR Primer, Index 19 (95% over 21bp)
CTCTTATACACATCTCCGAGCCCACGAGACGCGTTAAAATCTCGTATGCC27371.2021046713866588No Hit
ATACACATCTCCGAGCCCACGAGACGCGTTAAAATCTCGTATGCCGTCTT11590.5090388433091478No Hit
TCTTTATACACATCTCCGAGCCCACGAGACGCGTTAAAATCTCGTATGCC8520.37420284253614655No Hit
CTATACACATCTCCGAGCCCACGAGACGCGTTAAAATCTCGTATGCCGTC7510.32984311589747195RNA PCR Primer, Index 19 (95% over 22bp)
ACTTATACACATCTCCGAGCCCACGAGACGCGTTAAAATCTCGTATGCCG6860.30129477697159224No Hit
CTTATACACATCTCCGAGCCCACGAGACGCGATAAAATCTCGTATGCCGT3980.1748036752692328No Hit
CTTATACACATCTCCGAGCCCACGAGACGCGTTAAATCTCGTATGCCGTC3820.16777639184132395No Hit
CTTTATACACATCTCCGAGCCCACGAGACGCGTTAAAATCTCGTATGCCG3680.1616275188419037No Hit
CTTTACACATCTCCGAGCCCACGAGACGCGTTAAAATCTCGTATGCCGTC3560.15635705627097204No Hit
CTAATACACATCTCCGAGCCCACGAGACGCGTTAAAATCTCGTATGCCGT3240.14230248941515433RNA PCR Primer, Index 19 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACGCGTAAAAATCTCGTATGCCGT3090.13571441120148978No Hit
CTTAATACACATCTCCGAGCCCACGAGACGCGTTAAAATCTCGTATGCCG2640.11595017656049612No Hit
CTTATACACATCTCCGAGCCAACGAGACGCGTTAAAATCTCGTATGCCGT2620.11507176613200752RNA PCR Primer, Index 19 (95% over 21bp)
TTATACACATCTCCGAGCCCACGAGACGCGTTAAAATCTCGTATGCCGTC2580.11331494527503032RNA PCR Primer, Index 19 (95% over 22bp)
TATACACATCTCCGAGCCCACGAGACGCGTTAAAATCTCGTATGCCGTCT2490.10936209834683157No Hit
CTTATACACATCTCCGAGCCCACGAGACGCGTTAAAATCTCGTATGCAGT2360.10365243056165561No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGTAC150.002220836570.014063
ATATCGA150.002222772569.9986837
ACGTATG455.456968E-1269.9986840
ACTCCGA1050.066.66540511
AGGGGGG58900.064.5318970
AAACTCG1200.064.1654636
CGAATGC1200.064.1654641
AATGCCG1000.062.99881443
AACTCGA453.8380676E-1062.2210537
CTCGAAA352.9656803E-759.9988739
GACGAGT650.059.22965226
AACTCCG1250.058.7988910
AATCCGA308.359544E-658.33223711
TCGAATG1450.057.92994340
AACTCGT850.057.64597337
ATCTCGA1100.057.2716537
CGAGTTA553.6379788E-1157.2716528
ACGAGTT553.6379788E-1157.2716527
TGCCGTA553.6379788E-1157.2716545
GCCGTCA1800.056.3878346
Produced by FastQC (version 0.11.5)