FastQCFastQC Report
Wed 25 May 2016
SRR1781077_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781077_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences244120
Sequences flagged as poor quality0
Sequence length76
%GC66

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG8146833.37211207602818No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGGGTC150.002222954569.9987714
GGCAAAC200.006923509852.542131
TACGGGT200.00694599452.4990813
TAAGCGC200.00694599452.4990847
TCCGGTC200.00694599452.499088
TCGGTGG352.0828178E-549.9991245
TGGTCGG352.0828178E-549.9991249
GTCTACT451.6710364E-646.7041171
TATACCG305.870765E-446.665856
TACCGCC404.5846013E-543.7492338
CGGTGGT503.4700133E-641.99926446
TCGGCGG350.001253935539.99929452
GTGGTCG701.9179424E-839.99929448
ATACCGC350.001253935539.9992947
TGAACTA556.678363E-638.1811529
CTAGGTC556.678363E-638.1811533
CCAGGGT501.7067234E-434.99938614
ATCACGT400.00241592334.99938624
ACTAGGT601.2127779E-534.99938632
CTACGGA400.00241592334.9993863
Produced by FastQC (version 0.11.5)