FastQCFastQC Report
Wed 25 May 2016
SRR1781077_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781077_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences244120
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCCAAATAAATCTCGTATGCCGT4920020.154022611830246No Hit
TCTTATACACATCTCCGAGCCCACGAGACCCAAATAAATCTCGTATGCCG53902.2079305259708337No Hit
CATATACACATCTCCGAGCCCACGAGACCCAAATAAATCTCGTATGCCGT32751.3415533344256925No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCCAAATAAATCTCGTATGCC27531.1277240701294444No Hit
ATACACATCTCCGAGCCCACGAGACCCAAATAAATCTCGTATGCCGTCTT14800.6062592167786335No Hit
TCTTTATACACATCTCCGAGCCCACGAGACCCAAATAAATCTCGTATGCC11490.470670162215304No Hit
CTATACACATCTCCGAGCCCACGAGACCCAAATAAATCTCGTATGCCGTC10270.4206947402916599No Hit
CTTATACACATCTCCGAGCCCACGAGACACAAATAAATCTCGTATGCCGT8180.33508110765197446No Hit
ACTTATACACATCTCCGAGCCCACGAGACCCAAATAAATCTCGTATGCCG7280.2982139931181386No Hit
CTTTATACACATCTCCGAGCCCACGAGACCCAAATAAATCTCGTATGCCG4220.17286580370309684No Hit
CTTTACACATCTCCGAGCCCACGAGACCCAAATAAATCTCGTATGCCGTC3870.15852859249549403No Hit
TTATACACATCTCCGAGCCCACGAGACCCAAATAAATCTCGTATGCCGTC3600.1474684581353433No Hit
TCTTAATACACATCTCCGAGCCCACGAGACCCAAATAAATCTCGTATGCC3290.1347697853514665No Hit
CTAATACACATCTCCGAGCCCACGAGACCCAAATAAATCTCGTATGCCGT3160.12944453547435686No Hit
CTTATACACATCTCCGAGCCCACGAGACCCAAATAATCTCGTATGCCGTC3040.12452892020317877No Hit
CTTATACACATCTCCGAGCCAACGAGACCCAAATAAATCTCGTATGCCGT3040.12452892020317877No Hit
TATACACATCTCCGAGCCCACGAGACCCAAATAAATCTCGTATGCCGTCT2990.12248074717352121No Hit
CTTATACACATCTCCGAGCCCACGAGACCCAAATAAATCTCGTATGCAGT2900.11879403572013764No Hit
CTTATACACATCTCCGAGCCCACGAGACCCAAAAAAATCTCGTATGCCGT2850.1167458626904801No Hit
CTTAATACACATCTCCGAGCCCACGAGACCCAAATAAATCTCGTATGCCG2780.11387842044895953No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTTGAC150.00222344569.9948833
CGACTTA150.00222344569.9948848
AAACTCG1450.065.1676536
AGGGGGG62750.064.1387370
TGCCGAC1050.063.32869345
AACTCGT1150.060.86511237
CGTCTAC1500.060.66222848
AAGCCGT1050.059.99560544
CCGACTT1050.059.99560547
CGAATGC1350.059.6252741
ACTCCGA1300.059.22643311
GCCGACT1200.058.32906746
AATCACG900.058.32906336
AGCCGTC1100.057.2685445
TATGACG1850.056.752643
CATATAC9250.056.0303341
TCTCGAA1000.055.99590338
AGACCCG252.3881582E-455.99590325
AACTCGA509.767973E-1055.99590337
GACGCAA252.3881582E-455.99590326
Produced by FastQC (version 0.11.5)