FastQCFastQC Report
Wed 25 May 2016
SRR1781076_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781076_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences145190
Sequences flagged as poor quality0
Sequence length76
%GC76

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG7681252.90447000482127No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCTAGT150.00222115369.9986311
CGGTGGT207.906108E-569.9986346
TGGTCGC150.00222115369.9986349
TCGGTGG252.3842917E-455.99889445
GTTATGT252.3842917E-455.9988943
GGGTAGT200.00691207352.553281
ATCTCGG200.006940406752.4989742
GAACTAT200.006940406752.4989745
AGGCGTT200.006940406752.4989712
CTCGGTG200.006940406752.4989744
CTGCCGG200.006940406752.4989718
TCTCGGT200.006940406752.4989743
GGGCCCC200.006940406752.498979
CAAGAAC200.006940406752.4989734
CTAATAT200.006940406752.4989742
CCTCTAG305.833095E-446.7140241
CCTAAGA305.852963E-446.6818282
GTGGTCG305.862918E-446.6657548
GTGGGGC602.579327E-740.8325312
GGTCCTA350.001245914840.040591
Produced by FastQC (version 0.11.5)