FastQCFastQC Report
Wed 25 May 2016
SRR1781076_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781076_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences145190
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGCCGT4792833.010537915834426No Hit
TCTTATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGCCG50773.496797300089538No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGCC25531.7583855637440595No Hit
CATATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGCCGT22451.546249741717749No Hit
ATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGCCGTCTT10670.7348990977340037TruSeq Adapter, Index 7 (95% over 21bp)
TCTTTATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGCC10390.7156140230043392No Hit
CTATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGCCGTC8140.560644672498106No Hit
ACTTATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGCCG5420.37330394655279286No Hit
CTTATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTAGTATGCCGT4420.3044286796611337No Hit
CTTATACACATCTCCGAGCCCACGAGACCTTGGGTCATCACGTATGCCGT4310.29685240030305116No Hit
CTTATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGACGT4020.27687857290447004No Hit
CTTTATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGCCG3580.24657345547213996No Hit
CTTTACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGCCGTC3270.2252221227357256No Hit
CTTATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTAAGCCGT2810.19353949996556236No Hit
CTTATACACATCTCCGAGCCCACGAGACCTAGGGTCATCTCGTATGCCGT2800.19285074729664578No Hit
TCTTAATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGCC2670.18389696260073007No Hit
CTTAATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGCCG2580.17769818858048075No Hit
TTATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGCCGTC2470.17012190922239823No Hit
CTTATACACATCTCCGAGCCAACGAGACCTTGGGTCATCTCGTATGCCGT2330.16047937185756594No Hit
CTAATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGCCGT2220.15290309249948345No Hit
CCTTATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGCCG2180.15014808182381706No Hit
TATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGCCGTCT2050.14119429712790135No Hit
CTTATACACATCTCCGAGCCCACGAGACATTGGGTCATCTCGTATGCCGT2000.13775053378331842No Hit
CTTATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGCAGT1950.13430677043873546No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTTATA353.6743586E-970.022051
CCTATAG150.002218205270.022052
GGTGGCT150.002221239769.9979324
CAAATCG150.002221239769.9979336
AGCTAGA207.906495E-569.9979355
TGCAGAC150.002221239769.9979345
GCGTCAT150.002221239769.9979332
GACCTTC150.002221239769.9979326
CGAAGGC150.002221239769.9979334
ACCATGG850.065.8804127
AGGGGGG52350.065.7860270
ATGGGTC700.064.99808530
TGACGTA501.4551915E-1162.9981445
ATCTCGA950.062.62973437
GCCGTAA950.062.62973446
ATGCATG900.062.22038754
AACTCGT401.0586518E-861.2481937
GACCTAG1150.060.86776726
TATGCAT750.060.6648853
TCACCGA1100.060.4527611
Produced by FastQC (version 0.11.5)