FastQCFastQC Report
Wed 25 May 2016
SRR1781075_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781075_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences126350
Sequences flagged as poor quality0
Sequence length76
%GC91

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG9313873.71428571428571No Hit
TGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2140.16937079540957659No Hit
GTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1750.13850415512465375No Hit
GGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1670.13217253660466957No Hit
GGGGGGGGGGGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1480.11713494261970717No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTTGCG150.00222041169.9992119
CCTATCC150.00222041169.999219
TCGGCCC150.00222041169.9992136
TACACGT207.9018224E-569.999215
AGGTGTC150.00222041169.9992140
CTGCGGG150.00222041169.9992118
TATCCGG150.00222041169.9992111
AATAGAT150.00222041169.999215
ATCGGCC150.00222041169.9992135
CCCGACC150.00222041169.9992140
TGTAGGT301.016997E-769.9992137
AGGTCTC150.00222041169.9992140
GTAGGTC207.9018224E-569.9992138
ACCTATC207.9018224E-569.999218
GCCCGAC150.00222041169.9992139
TCTTCGC150.00222041169.9992153
ATACACG150.00222041169.999214
CGATCGG150.00222041169.9992133
GTGTAGG501.4551915E-1162.99928736
CGTCTGG453.8016879E-1062.221529
Produced by FastQC (version 0.11.5)