FastQCFastQC Report
Wed 25 May 2016
SRR1781075_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781075_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences126350
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTTATGAAAATCTCGTATGCCGT6043247.829046299960424No Hit
TCTTATACACATCTCCGAGCCCACGAGACTTATGAAAATCTCGTATGCCG66355.251286110011872No Hit
CATATACACATCTCCGAGCCCACGAGACTTATGAAAATCTCGTATGCCGT37312.952908587257618No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTTATGAAAATCTCGTATGCC33452.6474079936683816No Hit
ATACACATCTCCGAGCCCACGAGACTTATGAAAATCTCGTATGCCGTCTT14461.144440047487139RNA PCR Primer, Index 19 (95% over 23bp)
TCTTTATACACATCTCCGAGCCCACGAGACTTATGAAAATCTCGTATGCC12701.0051444400474872No Hit
CTATACACATCTCCGAGCCCACGAGACTTATGAAAATCTCGTATGCCGTC10840.8579343094578551No Hit
ACTTATACACATCTCCGAGCCCACGAGACTTATGAAAATCTCGTATGCCG8290.6561139691333597No Hit
CTTTATACACATCTCCGAGCCCACGAGACTTATGAAAATCTCGTATGCCG5610.44400474871388995No Hit
CTTTACACATCTCCGAGCCCACGAGACTTATGAAAATCTCGTATGCCGTC5240.4147210130589632RNA PCR Primer, Index 19 (95% over 21bp)
CTAATACACATCTCCGAGCCCACGAGACTTATGAAAATCTCGTATGCCGT4110.32528690146418676No Hit
TCTTAATACACATCTCCGAGCCCACGAGACTTATGAAAATCTCGTATGCC3470.27463395330431345No Hit
CTTATACACATCTCCGAGCCAACGAGACTTATGAAAATCTCGTATGCCGT3430.2714681440443213No Hit
CTTAATACACATCTCCGAGCCCACGAGACTTATGAAAATCTCGTATGCCG3390.26830233478432924No Hit
TTATACACATCTCCGAGCCCACGAGACTTATGAAAATCTCGTATGCCGTC3340.26434507320933914No Hit
CTTATACACATCTCCGAGCCCACGAGACTAATGAAAATCTCGTATGCCGT3270.258804907004353No Hit
CTTATACACATCTCCGAGCCCACGAGACTTATGAAAATCTCGTATGCAGT3200.25326474079936684No Hit
CTTATACACATCTCCGAGCCCACGAGACTTATGAAAATCTCGTATGACGT3170.2508903838543728No Hit
CTTATACACATCTCCGAGCCCACGAGACTTATGAAATCTCGTATGCCGTC3050.2413929560743965No Hit
TATACACATCTCCGAGCCCACGAGACTTATGAAAATCTCGTATGCCGTCT2700.21369212504946575RNA PCR Primer, Index 19 (95% over 22bp)
CCTTATACACATCTCCGAGCCCACGAGACTTATGAAAATCTCGTATGCCG2580.20419469726948952No Hit
CTTATACACATCTCCGAGCCCACGAGACATATGAAAATCTCGTATGCCGT2570.2034032449544915No Hit
CTTATACACATCTCCGAGCCCACGAGACTTAAGAAAATCTCGTATGCCGT2450.19390581717451524No Hit
CTTATACACATCTCCGAGACCACGAGACTTATGAAAATCTCGTATGCCGT2310.18282548476454294No Hit
CTTAAACACATCTCCGAGCCCACGAGACTTATGAAAATCTCGTATGCCGT2040.16145627225959636No Hit
CTTATACACATCACCGAGCCCACGAGACTTATGAAAATCTCGTATGCCGT2010.1590819153146023No Hit
TCTATACACATCTCCGAGCCCACGAGACTTATGAAAATCTCGTATGCCGT1760.13929560743965178No Hit
CAAATACACATCTCCGAGCCCACGAGACTTATGAAAATCTCGTATGCCGT1630.12900672734467747No Hit
CTTATACACATCTCAGAGCCCACGAGACTTATGAAAATCTCGTATGCCGT1530.12109220419469727No Hit
ACTTTATACACATCTCCGAGCCCACGAGACTTATGAAAATCTCGTATGCC1450.1147605856747131No Hit
CTTATACACATCTCCGAGCACACGAGACTTATGAAAATCTCGTATGCCGT1340.10605461020973486No Hit
CATATACACATCACCGAGCCCACGAGACTTATGAAAATCTCGTATGCCGT1320.10447170557973881No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACGTC150.002210811770.0756766
TATACGC150.002210811770.0756763
TACACGT150.002210811770.0756765
TTATACG150.002210811770.0756762
TACGCAT150.002210811770.0756765
ATACGCA150.002210811770.0756764
AGCGCGA150.002210811770.0756765
TCCTTAT150.002214289570.047921
CCTATAC150.002214289570.047921
GCGACGT150.002217771570.0201958
CACGTCT150.002217771570.0201957
GCATCTC150.002217771570.0201958
CTCGAAA150.002221257469.99248539
ACCGGTC150.002221257469.99248564
AGGGGGT207.905582E-569.99248570
TCCCACG150.002221257469.99248518
TGACCCG150.002221257469.99248554
CGCCTGA150.002221257469.99248550
ACGTCGG150.002221257469.99248511
TCGAAAG207.905582E-569.99248540
Produced by FastQC (version 0.11.5)