FastQCFastQC Report
Wed 25 May 2016
SRR1781074_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781074_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences386161
Sequences flagged as poor quality0
Sequence length76
%GC61

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG9448024.466479007460617No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGATTA200.006949016452.49912642
TACCCGC352.0850828E-549.9991711
TCGGTGG855.638867E-1141.17578545
GGGATCC350.001254836239.99933633
TGGCGCT1350.033.70314413
CGGTGGT853.9505594E-628.8230546
TTAGGTG500.007205119427.99953525
GGATCCA500.007205119427.99953534
CGCCGAC500.007205119427.99953515
CTCGGTG755.5792378E-527.99953344
TATTAGA657.938467E-426.9296022
AACCACG657.95049E-426.9226267
CGATTTC657.95049E-426.92262616
GCGTTAT657.95049E-426.92262611
GCCCTCG808.6570275E-526.24956320
GGCGCGA700.001225198624.99958417
GGCCGTT750.001831015123.33294315
GTGGCGC901.925865E-423.33294312
TACATAC750.001831015123.3329433
TTCCCAA750.001831015123.33294323
Produced by FastQC (version 0.11.5)