FastQCFastQC Report
Wed 25 May 2016
SRR1781074_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781074_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences386161
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTTTAATGCATCTCGTATGCCGT6202216.06117655589249No Hit
TCTTATACACATCTCCGAGCCCACGAGACTTTAATGCATCTCGTATGCCG76471.9802621186499934No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTTTAATGCATCTCGTATGCC35950.9309588487703315No Hit
CATATACACATCTCCGAGCCCACGAGACTTTAATGCATCTCGTATGCCGT19920.5158470171767734No Hit
ATACACATCTCCGAGCCCACGAGACTTTAATGCATCTCGTATGCCGTCTT17620.45628636760315Illumina PCR Primer Index 3 (95% over 21bp)
TCTTTATACACATCTCCGAGCCCACGAGACTTTAATGCATCTCGTATGCC15690.4063072138305007No Hit
CTATACACATCTCCGAGCCCACGAGACTTTAATGCATCTCGTATGCCGTC11760.30453619086339634No Hit
CTTTACACATCTCCGAGCCCACGAGACTTTAATGCATCTCGTATGCCGTC5240.13569469728947253No Hit
CTTTATACACATCTCCGAGCCCACGAGACTTTAATGCATCTCGTATGCCG5000.1294796729861379No Hit
ACTTATACACATCTCCGAGCCCACGAGACTTTAATGCATCTCGTATGCCG4450.11523690895766274No Hit
CTTATACACATCTCCGAGCCAACGAGACTTTAATGCATCTCGTATGCCGT4190.10850396596238357No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCT150.002223978569.9984414
AGGGGGG77350.064.8854470
GCCGTAT900.062.2208446
GCCGACT750.060.6653246
CGAATGC1150.057.824841
GACGAAT252.389305E-455.99875346
ACGAATT252.389305E-455.99875347
CGTATGA2400.055.41543641
CTCGTAT101400.053.5688339
TCGTATG102200.053.49196640
CGTATGC100350.053.43179741
CATCTCG101800.053.42710536
ATCTCGT102050.053.39910537
GCCGTCT98250.053.39830446
TCTCGTA102250.053.3973538
CCGTCTT97800.053.3577147
TGCCGTA1050.053.3321545
TATGCCG99600.053.3070543
GCATCTC102400.053.2849535
GTATGCC99550.053.2635142