FastQCFastQC Report
Wed 25 May 2016
SRR1781073_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781073_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences760432
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG9741112.809955393776171No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGCGT150.002224713669.999215
CGCGTCT352.0869436E-549.9994357
GCGCGTC459.199574E-538.888456
TAAGACG450.00430779231.1107666
TCGTTGT703.489575E-529.99966234
CATCGTT604.983055E-429.16633632
CGGTAAG1202.2992026E-929.16633628
GTCGCCG500.007209254427.99968751
CGTGCGA500.007209254427.99968710
CCGTGCG500.007209254427.9996879
CTTGCGG1253.5579433E-927.99968739
CGGTACT1253.5579433E-927.99968743
CCTTGCG1253.5579433E-927.99968738
TGGCGGC755.5860488E-527.99968319
TAAGCGT755.5860488E-527.99968310
TCCGGCG1403.947207E-1027.49969122
CGTTCGG1305.4042175E-926.92277324
CGGGGTC1455.9480953E-1026.55142427
GCCCGAT808.667549E-526.2497047
TTCGGTA1358.079951E-925.92563226
Produced by FastQC (version 0.11.5)