FastQCFastQC Report
Wed 25 May 2016
SRR1781073_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781073_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences760432
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCTCATGGGATCTCGTATGCCGT627988.258200601763209No Hit
TCTTATACACATCTCCGAGCCCACGAGACCTCATGGGATCTCGTATGCCG72700.9560355166536916No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCTCATGGGATCTCGTATGCC32600.4287036842216004No Hit
CATATACACATCTCCGAGCCCACGAGACCTCATGGGATCTCGTATGCCGT19070.2507785048498748No Hit
ATACACATCTCCGAGCCCACGAGACCTCATGGGATCTCGTATGCCGTCTT16970.22316262335093737RNA PCR Primer, Index 34 (95% over 23bp)
TCTTTATACACATCTCCGAGCCCACGAGACCTCATGGGATCTCGTATGCC14700.19331117049256213No Hit
CTATACACATCTCCGAGCCCACGAGACCTCATGGGATCTCGTATGCCGTC12200.16043512108906516No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACGTAT150.002224912469.99763546
TGACGTA301.02405465E-769.99763545
TCGTAAT150.002224912469.99763540
AGGGGGG79350.061.2203670
AGCCGTC1500.058.3313645
CGAATGC1100.057.2707941
TATGACG2250.055.99810443
CGACTTA252.3909201E-455.99810448
CGTATGA1950.055.63914541
GCCGACT900.054.44260446
TGCCGTA1750.053.99817345
TCTCGTA98650.053.2521338
GCCGTCT94450.053.0633246
CTCGTAT97450.053.0459239
TCGTATG99400.052.9911740
CCGTCTT94250.052.95311747
CGTATGC98300.052.94327541
CGTCTTC93150.052.9021348
TATGCCG97850.052.86484543
GGATCTC99600.052.84961735