FastQCFastQC Report
Wed 25 May 2016
SRR1781072_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781072_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences496017
Sequences flagged as poor quality0
Sequence length76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG9463919.079789603985347No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTCGC506.192931E-848.99908449
TCGGTGG1250.036.3993245
CTCGGTG1300.034.99934444
TCTCGGT1201.8189894E-1234.99934443
CGGTGGT1003.255991E-1034.99934446
CGCGTGT501.7092725E-434.9993447
CGTATCA552.9913476E-431.81758956
GTACTAG450.0042874331.1387791
TCGCCGT450.004306518531.11052952
CCGTGCG450.004306518531.1105299
CGAGAAT450.004306518531.11052914
GTCGCCG802.4652327E-630.62442651
TGCGCGT604.9808016E-429.166125
GGTTCGG604.9808016E-429.1661210
CGTCAGA755.5671488E-528.010772
GGTACCG500.00720713827.9994757
TCCGAGC500.00720713827.99947536
TACGGTG500.00720713827.99947528
GCCGTTA500.00720713827.99947516
TACCGCG500.00720713827.9994759
Produced by FastQC (version 0.11.5)