FastQCFastQC Report
Wed 25 May 2016
SRR1781072_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781072_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences496017
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACACCGGCTAATCTCGTATGCCGT6115212.328609704909308No Hit
TCTTATACACATCTCCGAGCCCACGAGACACCGGCTAATCTCGTATGCCG70161.4144676492942783No Hit
CTCTTATACACATCTCCGAGCCCACGAGACACCGGCTAATCTCGTATGCC35830.7223542741478618No Hit
CATATACACATCTCCGAGCCCACGAGACACCGGCTAATCTCGTATGCCGT20440.41208265039303094No Hit
TCTTTATACACATCTCCGAGCCCACGAGACACCGGCTAATCTCGTATGCC16470.3320450710358718No Hit
ATACACATCTCCGAGCCCACGAGACACCGGCTAATCTCGTATGCCGTCTT16240.32740813318898343TruSeq Adapter, Index 10 (95% over 21bp)
CTATACACATCTCCGAGCCCACGAGACACCGGCTAATCTCGTATGCCGTC10720.21612162486366396No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG78050.064.1225170
CGTCTAC1150.060.8666748
CCGGCAA1450.060.34195730
CGGATAA1050.059.9971531
ACGTATG1050.056.66397540
CGACTTA252.3902686E-455.9973448
AACGTAT452.7004717E-854.44185339
AATCTCG102450.052.98430636
CGAATGC1850.052.9704641
CGTATGC101650.052.71269641
CTCGTAT101350.052.69606439
TATGCCG99850.052.68152243
TCTCGTA101850.052.6779138
GCCGTCT98850.052.6125746
TCGTATG103750.052.5902740
ATGCCGT100450.052.575944
CCGTCTT97900.052.55112547
GTACGGA200.006937179752.5239756
CGTCTTC99250.052.50631748
ACCGGAT1000.052.49750529