FastQCFastQC Report
Wed 25 May 2016
SRR1781071_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781071_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences405981
Sequences flagged as poor quality0
Sequence length76
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG8954222.05571196681618No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGGAC150.002224066869.998443
TCGGAGT150.002224066869.998438
CGCGTCT150.002224066869.99847
TCGTTTC200.006949442452.49880245
GCAAGTC200.006949442452.4988026
ACGGTGC200.006949442452.49880231
TGTGCGC305.875548E-446.66563
GTGCGCG404.5901492E-543.7494
TACTCGA350.001251912940.0188032
ATCCGAG350.001254950739.99908438
TGCGCGT350.001254950739.9990845
TTATCGC459.192625E-538.88845
TAGCTCG556.689561E-638.18094310
CGAGTAT400.002417867534.99920341
GACAATT400.002417867534.99920364
CGTGTTA501.7087688E-434.999234
GTTTCGG652.1010184E-532.30695321
AGCTCGT652.1010184E-532.30695311
GATCTTA450.004282383231.1449241
AATCCGA450.00430569431.110437
Produced by FastQC (version 0.11.5)