FastQCFastQC Report
Wed 25 May 2016
SRR1781071_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781071_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences405981
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGCCGT5703014.047455422790723No Hit
TCTTATACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGCCG67391.6599298981972062No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGCC33060.8143238230360533No Hit
CATATACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGCCGT15710.38696392195693885No Hit
ATACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGCCGTCTT14250.3510016478603678RNA PCR Primer, Index 14 (95% over 21bp)
TCTTTATACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGCC13080.3221825651939376No Hit
CTATACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGCCGTC10170.2505043339466625No Hit
CTTTATACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGCCG4950.12192688820412778No Hit
CTTTACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGCCGTC4710.11601528150332159No Hit
CTTATACACATCTCCGAGCCCACGAGACCGGTTACCATCTCGTATGCCGT4160.10246784948064072No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTCAA252.8389877E-669.9945847
AACTCGA207.9254896E-569.99457637
AGGGGGG70250.064.01639670
ACCGGTA700.059.99535427
CGTCAAC352.972738E-759.99535448
GACGTCA308.3742125E-658.3288246
GTTACCA1250.055.99566331
CCGACTT700.054.99574347
CGTATGA2300.054.7783741
CGAATGC850.053.5252741
GCCGTCA2300.053.2567546
TCTCGTA90800.053.11262538
CATCTCG89550.053.1114736
CTCGTAT90300.053.09666439
TATGCCG89050.053.0952743
TCGTATG91550.053.05979540
GCCGTCT86850.053.0298646
CGTATGC89600.053.00370841
ATGCCGT88750.052.99871444
ATCTCGT90750.052.9490737