FastQCFastQC Report
Wed 25 May 2016
SRR1781070_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781070_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences294333
Sequences flagged as poor quality0
Sequence length76
%GC66

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG9428532.033445111489364No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCATT150.00222334169.9993256
CGCCAAT150.00222334169.9993251
CGACGTG200.006947192452.499499
CATAACG200.006947192452.4994953
TATACGC200.006947192452.499493
TGCCGGC407.46566E-752.4994919
TGTGCGC352.0837764E-549.999513
TGCGCGT352.0837764E-549.999515
CGCGTCT305.8725174E-446.6662147
GTGCGCG451.6816011E-646.666214
ATCCTCG404.586701E-543.74957731
CTACAGA350.001251166140.0200081
TCCACCG350.001253259640.006412
GCGCATC350.001254307539.999613
ATCGGTT350.001254307539.9996122
GATTAAC350.001254307539.9996133
AATCGGC350.001254307539.9996115
CGGCGAT459.1857495E-538.88850815
GCGATAT459.1857495E-538.88850817
GATATTA501.7023855E-435.0175061
Produced by FastQC (version 0.11.5)