FastQCFastQC Report
Wed 25 May 2016
SRR1781070_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781070_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences294333
Sequences flagged as poor quality0
Sequence length76
%GC48

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTCGTATGCCGT240868.18324822564918No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTAGTATGCCGT61152.0775787968049793No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCGCGTATGCCGT19990.6791627170585697No Hit
TCTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTCGTATGCCG17640.5993211770341755No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCGAGTATGCCGT12250.41619526182928857No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTCGTATGCCGG10880.3696493427512375No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTCGTATTCCGT10770.3659120791756276No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTAGTATTCCGT7870.2673842212731838No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTCGTATGCC7820.265685465102452No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTCGGATGCCGT7370.2503966595658659No Hit
TCTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTAGTATGCCG7270.2469991472244023No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTCGTATGACGT6120.20792775529757113No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTCGTATGCCCT5680.19297870099513137No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTCGTATGGCGT5170.17565138805366712No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTCGTAAGCCGT5120.17395263188293533No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCGCGTATGCCGG4730.16070233375122736No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTAGTATGACGT4200.14269551834147037No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTCGTTTGCCGT3850.13080422514634785No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTAGTATGCCGG3700.1257079566341525No Hit
TCTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCGCGTATGCCG3600.1223104442926889No Hit
TCTTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTCGTATGCC3530.11993218565366438No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCGCGGATGCCGT3470.11789367824878624No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCGAGTATGCCGG2960.10056636530732198No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTCGCG500.069.9967828
AATGCGA207.920643E-569.9967744
GACGGCT353.7016434E-969.9967746
ATGCGAA207.920643E-569.9967745
AACTCGA207.920643E-569.9967737
CAACTCG207.920643E-569.9967736
CGCTTAA150.002223661969.9967749
CGACTTA455.456968E-1269.99676548
AGGGGGG50650.065.9890670
CGTCTTC11700.063.71500848
GACGTAT900.062.2193546
GCTTAAG900.062.2193550
CGCGTAA453.8380676E-1062.2193539
GTCTTCT10550.062.03505349
CCGTCTT30000.061.9471447
GCCGTCT28800.061.61173646
TCTTCTG10600.061.41226650
ACGGCTT401.0639269E-861.24717747
CCGACTT401.0639269E-861.24717747
TAAGCGG401.0639269E-861.24717729
Produced by FastQC (version 0.11.5)