FastQCFastQC Report
Wed 25 May 2016
SRR1781069_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781069_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences217743
Sequences flagged as poor quality0
Sequence length76
%GC69

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG8468938.89401725887859No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGAAACG150.002222649569.9986323
CATGTCG150.002222649569.9986311
GACAGCG252.3869511E-455.99889829
CGTATCA252.3869511E-455.99889856
TCGGTGG452.6904672E-854.44337545
CTTGTGC200.006926137552.5351681
TTATGCT200.006945047552.4989750
AACGAAC200.006945047552.4989736
CCGGTGA200.006945047552.4989736
GCCGTAT200.006945047552.4989754
CGCGTCT352.0820937E-549.9990167
GCGCGTC305.869419E-446.665756
AGACGCT305.869419E-446.6657561
TGCGCGT305.869419E-446.665755
CTCGGTG451.6796839E-646.6657544
CGGTGGT604.9094524E-946.6657546
TCTCGGT404.5830147E-543.7491443
TGGTCGC350.001253649739.99921449
TTGTGCG459.153586E-538.9059942
GTGGTCG556.6751345E-638.18106548
Produced by FastQC (version 0.11.5)