FastQCFastQC Report
Wed 25 May 2016
SRR1781069_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781069_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences217743
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGACGCTCCATCTCGTATGCCGT5487525.201728643400706No Hit
TCTTATACACATCTCCGAGCCCACGAGACGACGCTCCATCTCGTATGCCG63952.9369486045475632No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGACGCTCCATCTCGTATGCC32441.4898297534249092No Hit
CTTATACACATCTCCGAGCCCACGAGACGCCGCTCCATCTCGTATGCCGT16460.7559370450485204No Hit
CATATACACATCTCCGAGCCCACGAGACGACGCTCCATCTCGTATGCCGT15790.7251668251103365No Hit
ATACACATCTCCGAGCCCACGAGACGACGCTCCATCTCGTATGCCGTCTT14220.6530634739119053Illumina PCR Primer Index 11 (95% over 22bp)
TCTTTATACACATCTCCGAGCCCACGAGACGACGCTCCATCTCGTATGCC11790.541464019509238No Hit
CTATACACATCTCCGAGCCCACGAGACGACGCTCCATCTCGTATGCCGTC9100.41792388274249914No Hit
CTTTACACATCTCCGAGCCCACGAGACGACGCTCCATCTCGTATGCCGTC4500.20666565630123584No Hit
CTTTATACACATCTCCGAGCCCACGAGACGACGCTCCATCTCGTATGCCG4290.1970212590071782No Hit
ACTTATACACATCTCCGAGCCCACGAGACGACGCTCCATCTCGTATGCCG4160.19105091782514247No Hit
CTTATACACATCTCCGAGCCAACGAGACGACGCTCCATCTCGTATGCCGT4020.18462131962910402No Hit
TCTTAATACACATCTCCGAGCCCACGAGACGACGCTCCATCTCGTATGCC3380.1552288707329283No Hit
CTTATACACATCTCCGAGCCCACGAGACGACGCTCAATCTCGTATGCCGT3150.1446659594108651No Hit
CTTAATACACATCTCCGAGCCCACGAGACGACGCTCCATCTCGTATGCCG3060.14053264628484038No Hit
CTTATACACATCTCCGAGCCCACGAGACGACGCTCCATCTCGTATGACGT2850.13088824899078272No Hit
TATACACATCTCCGAGCCCACGAGACGACGCTCCATCTCGTATGCCGTCT2700.12399939378074151Illumina PCR Primer Index 11 (95% over 21bp)
TTATACACATCTCCGAGCCCACGAGACGACGCTCCATCTCGTATGCCGTC2640.12124385169672504No Hit
CTTATACACATCTCCGAGACCACGAGACGACGCTCCATCTCGTATGCCGT2600.11940682364071406No Hit
CTTATACACATCTCCGAGCCCACGAGACGACGCTCCATCTCGTATGCAGT2470.11343648245867835No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACGA150.002223025869.99563624
AATGACG353.6961865E-969.99563643
TCGAAAG150.002223025869.99563640
AGGGGGG65900.065.0035470
ATATACG352.9659714E-759.9962610
ACGCTAC1050.059.9962630
ATACGAG352.9659714E-759.9962612
GACGCTA1000.059.49629229
TCGAATG1250.058.79633340
ACCGAGA308.360481E-658.32969713
TGACGAC308.360481E-658.32969745
AAGCCGT601.8189894E-1258.32969744
ATGACGA308.360481E-658.32969744
CGCTACA1100.057.26915731
TCTACGA800.056.87145611
TCTTTAT2350.056.618231
CACCGAG1300.056.53493512
AACGAGC252.3874546E-455.9965113
CGACTAA252.3874546E-455.9965148
TCTCGAA950.055.25971638
Produced by FastQC (version 0.11.5)