FastQCFastQC Report
Wed 25 May 2016
SRR1781068_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781068_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences258003
Sequences flagged as poor quality0
Sequence length76
%GC66

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG8816634.1724708627419No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGACGC150.002223090369.9988312
GCCGTTG308.362584E-658.33236328
CGATTAG252.3877466E-455.9990752
TGCGATT252.3877466E-455.9990750
GTACTCG252.3877466E-455.9990759
CGGTGTA252.3877466E-455.9990755
TTGCGAT252.3877466E-455.9990749
TACTCGT252.3877466E-455.9990760
CGTAAGG252.3877466E-455.999073
TCGGTGT252.3877466E-455.9990754
GTTTGCG200.00694641552.49912658
GCGGTTT200.00694641552.49912662
AAGGTCC200.00694641552.49912634
TAGCCGG200.00694641552.49912628
TCGGCTT200.00694641552.49912614
CGCGTGT200.00694641552.4991267
CGTTGAG352.0831385E-549.99917230
GTAGTCG305.871363E-446.6658950
CGTTCGG404.585306E-543.7492724
TAGCCGT404.585306E-543.7492726
Produced by FastQC (version 0.11.5)