FastQCFastQC Report
Wed 25 May 2016
SRR1781068_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781068_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences258003
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGGAGTAAGATCTCGTATGCCGT5868722.74663472905354No Hit
TCTTATACACATCTCCGAGCCCACGAGACGGAGTAAGATCTCGTATGCCG68252.645318077696771No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGGAGTAAGATCTCGTATGCC31261.2116138184439715No Hit
CATATACACATCTCCGAGCCCACGAGACGGAGTAAGATCTCGTATGCCGT20110.7794483009887482No Hit
ATACACATCTCCGAGCCCACGAGACGGAGTAAGATCTCGTATGCCGTCTT13630.5282884307546811TruSeq Adapter, Index 9 (95% over 21bp)
TCTTTATACACATCTCCGAGCCCACGAGACGGAGTAAGATCTCGTATGCC12460.482940120851308No Hit
CTATACACATCTCCGAGCCCACGAGACGGAGTAAGATCTCGTATGCCGTC10090.3910807238675519No Hit
CTTTACACATCTCCGAGCCCACGAGACGGAGTAAGATCTCGTATGCCGTC5300.20542396793835732No Hit
ACTTATACACATCTCCGAGCCCACGAGACGGAGTAAGATCTCGTATGCCG4380.16976546784339716No Hit
CTTATACACATCTCCGAGCCCACGAGACGGAGAAAGATCTCGTATGCCGT4350.16860269066638758No Hit
CTTATACACATCTCCGAGCCAACGAGACGGAGTAAGATCTCGTATGCCGT4280.16588954392003194No Hit
CTTTATACACATCTCCGAGCCCACGAGACGGAGTAAGATCTCGTATGCCG4200.16278880478133975No Hit
TTATACACATCTCCGAGCCCACGAGACGGAGTAAGATCTCGTATGCCGTC3430.13294419057142745No Hit
CTAATACACATCTCCGAGCCCACGAGACGGAGTAAGATCTCGTATGCCGT3420.13255659817909093No Hit
TCTTAATACACATCTCCGAGCCCACGAGACGGAGTAAGATCTCGTATGCC3400.1317814133944179No Hit
CTTATACACATCTCCGAGACCACGAGACGGAGTAAGATCTCGTATGCCGT3380.13100622860974487No Hit
CTTAATACACATCTCCGAGCCCACGAGACGGAGTAAGATCTCGTATGCCG3070.11899086444731263No Hit
CTTATACACATCTCAGAGCCCACGAGACGGAGTAAGATCTCGTATGCCGT2880.11162660899291868No Hit
CTTATACACATCTCCGAGCCCACGAGACGGAGTAAGCTCTCGTATGCCGT2790.10813827746188998No Hit
CCTTATACACATCTCCGAGCCCACGAGACGGAGTAAGATCTCGTATGCCG2750.1065879078925439No Hit
CTTATACACATCTCCGAGCCCACGAGACGGAGTAAGATCTCGTATGCAGT2670.1034871687538517No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAATTCG150.00221999170.023451
AAATCCG252.8323702E-670.0098910
ATAACCG150.00222169970.0098810
ACACGGA207.919049E-569.99631525
ATCGTAA150.002223407869.99631539
ATATCGA150.002223407869.99631537
TACGCAG150.002223407869.99631523
AGGGGGG73050.064.58250470
TGACGAC401.0631993E-861.24677745
GACGACT352.967954E-759.9968446
GCCGTAT352.967954E-759.9968446
TGACGTA308.36437E-658.33026545
ATACGAG252.3858849E-456.00790812
GACACGG252.3881714E-455.99705524
GACGTAT252.3881714E-455.99705546
CGAATGC1500.055.99705541
AACTCGA252.3881714E-455.99705537
ACGGAGA2250.055.9970527
CATATAC5950.055.312651
TCACCGA1900.055.27096611
Produced by FastQC (version 0.11.5)