FastQCFastQC Report
Wed 25 May 2016
SRR1781067_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781067_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences502802
Sequences flagged as poor quality0
Sequence length76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG9414518.724070309982856No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCATTG200.006950048352.49947743
GGACCTA404.575222E-543.775681
CGTGCGA701.9248546E-839.99960310
TGCGCGT350.001255156539.9996035
ACGGTCA350.001255156539.99960311
CGTATCA459.195054E-538.88850456
GCCGTAT459.195054E-538.88850454
TCGGTGG1151.8189894E-1236.52137845
TGTGCGC501.7092176E-434.9996533
TCAATGC501.7092176E-434.99965315
AGCCTTA501.7092176E-434.9996536
TGGTCGC708.687657E-734.99965349
TGGGCGC552.9912518E-431.8178673
GCGCTAT552.9912518E-431.81786719
CCGTGCG1001.2574674E-831.4996879
GCGAGAA901.7555249E-731.11080413
TCGCCGT450.00430638931.11080452
GGTATAC450.00430638931.1108043
ATCGGTT450.00430638931.11080422
TATCGGT450.00430638931.11080421
Produced by FastQC (version 0.11.5)