FastQCFastQC Report
Wed 25 May 2016
SRR1781067_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781067_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences502802
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCATCCTGTATCTCGTATGCCGT5754311.444465216924355No Hit
TCTTATACACATCTCCGAGCCCACGAGACCATCCTGTATCTCGTATGCCG64541.2836066682312322No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCATCCTGTATCTCGTATGCC32260.6416044486696553No Hit
CTTATACACATCTCCGAGCCCACGAGACCATCCTGTATCTAGTATGCCGT22710.45166884777705735No Hit
CATATACACATCTCCGAGCCCACGAGACCATCCTGTATCTCGTATGCCGT12870.2559655689515953No Hit
TCTTTATACACATCTCCGAGCCCACGAGACCATCCTGTATCTCGTATGCC12610.25079454735661355No Hit
ATACACATCTCCGAGCCCACGAGACCATCCTGTATCTCGTATGCCGTCTT12060.2398558478287676TruSeq Adapter, Index 2 (95% over 21bp)
CTATACACATCTCCGAGCCCACGAGACCATCCTGTATCTCGTATGCCGTC8290.16487603470153261No Hit
CTTATACACATCTCCGAGCCCACGAGACAATCCTGTATCTCGTATGCCGT7550.1501585117004308No Hit
CTTATACACATCTCCGAGCCCACGAGACCATCCTGTATCTCGTATGACGT5520.10978476617038119No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACTTA207.9254E-569.9980248
ACGTATG150.00222442569.9980140
AGGGGGG68150.063.8353170
AATCCGT252.3900751E-455.9984144
CTGGTCG252.3900751E-455.998419
CGTCTTC71600.055.77355648
GCCGTCT80300.055.65844746
CTGCTTG70200.055.48987654
CCGTCTT80300.055.44051747
GTCTTCT69750.055.34609649
TATGCCG90350.055.1616943
TCTTCTG70050.055.1590350
TGCCGTC86750.055.0704845
TCGTATG88050.055.01263840
ATGCCGT90500.054.91556544
CGTATGC86750.054.90910341
CTTGAAA85150.054.83109357
CTCGTAT88100.054.7827839
TCTCGTA89000.054.74001738
CTTCTGC70400.054.63623451