FastQCFastQC Report
Wed 25 May 2016
SRR1781066_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781066_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences221665
Sequences flagged as poor quality0
Sequence length76
%GC72

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG9548543.076263731306256No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCATC252.3869287E-455.99945411
CTCGGTG407.45722E-752.49949344
CGGTGTA200.00694493952.49949334
TCTCGGT451.6797057E-646.66621443
TCGGTGG659.962605E-943.07650445
CGGTGGT701.9164872E-839.99961546
CTGGCGC350.001253636839.99961512
TGGACAG400.002415351634.999665
GGTGGTC601.2122098E-534.9996647
CCGAATG400.002415351634.9996627
GCGCTGC501.7061386E-434.9996615
TGGTCGC400.002415351634.9996649
TGCCGGC601.2122098E-534.9996619
GGCGCTG652.0965532E-532.30737714
GTCTCGG552.9858894E-431.81787742
CAGTTAG450.00430123831.1108120
GCCGGCG901.7480124E-731.1108120
GCCTGAT450.00430123831.1108112
ATCTTGT450.00430123831.110816
TCTGGCG450.00430123831.1108111
Produced by FastQC (version 0.11.5)