FastQCFastQC Report
Wed 25 May 2016
SRR1781066_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781066_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences221665
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGCGTTAAAATCTCGTATGCCGT6348528.640064962894456No Hit
TCTTATACACATCTCCGAGCCCACGAGACGCGTTAAAATCTCGTATGCCG75683.414161008729389No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGCGTTAAAATCTCGTATGCC33921.53023706945165No Hit
CATATACACATCTCCGAGCCCACGAGACGCGTTAAAATCTCGTATGCCGT21870.9866239595786435RNA PCR Primer, Index 19 (95% over 21bp)
TCTTTATACACATCTCCGAGCCCACGAGACGCGTTAAAATCTCGTATGCC13930.6284257776374259No Hit
ATACACATCTCCGAGCCCACGAGACGCGTTAAAATCTCGTATGCCGTCTT13890.6266212527913744No Hit
CTATACACATCTCCGAGCCCACGAGACGCGTTAAAATCTCGTATGCCGTC10620.47910134662666637RNA PCR Primer, Index 19 (95% over 22bp)
CTTATACACATCTCCGAGCCCACGAGACGCGTTAAATCTCGTATGCCGTC6380.28782171294521014No Hit
ACTTATACACATCTCCGAGCCCACGAGACGCGTTAAAATCTCGTATGCCG5250.23684388604425596No Hit
CTTTACACATCTCCGAGCCCACGAGACGCGTTAAAATCTCGTATGCCGTC5030.22691899939097288No Hit
CTTATACACATCTCCGAGCCCACGAGACGCGATAAAATCTCGTATGCCGT4870.21970090000676695No Hit
CTTATACACATCTCCGAGCCAACGAGACGCGTTAAAATCTCGTATGCCGT4810.21699411273768976RNA PCR Primer, Index 19 (95% over 21bp)
CTTTATACACATCTCCGAGCCCACGAGACGCGTTAAAATCTCGTATGCCG4360.1966932082196107No Hit
TTATACACATCTCCGAGCCCACGAGACGCGTTAAAATCTCGTATGCCGTC3840.1732343852209415RNA PCR Primer, Index 19 (95% over 22bp)
TCTTAATACACATCTCCGAGCCCACGAGACGCGTTAAAATCTCGTATGCC3640.16421176099068413No Hit
CTTATACACATCTCCGAGACCACGAGACGCGTTAAAATCTCGTATGCCGT3480.15699366160647824RNA PCR Primer, Index 19 (95% over 21bp)
CTTAATACACATCTCCGAGCCCACGAGACGCGTTAAAATCTCGTATGCCG3480.15699366160647824No Hit
CTTATACACATCTCCGAGCCCACGAGACGCGTAAAAATCTCGTATGCCGT3140.1416552004150407No Hit
CCTTATACACATCTCCGAGCCCACGAGACGCGTTAAAATCTCGTATGCCG2790.12586560801209032No Hit
CTTATACACATCTCCGAGCCCACGAGACGCGTTAAAATCTCGTATGCAGT2760.12451221437755171No Hit
CTTATACACATCTCCGAGCCCACGAGACGCGTTAAAATCTCGTATGACGT2680.12090316468544876No Hit
CTAATACACATCTCCGAGCCCACGAGACGCGTTAAAATCTCGTATGCCGT2640.1190986398393973RNA PCR Primer, Index 19 (95% over 21bp)
TATACACATCTCCGAGCCCACGAGACGCGTTAAAATCTCGTATGCCGTCT2610.11774524620485867No Hit
CTTATACACATCTCAGAGCCCACGAGACGCGTTAAAATCTCGTATGCCGT2600.1172941149933458RNA PCR Primer, Index 19 (95% over 21bp)
CTTATACACATCTCCGAGCACACGAGACGCGTTAAAATCTCGTATGCCGT2390.10782035955157558RNA PCR Primer, Index 19 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTTTCG150.0022228769.99739
ACTAATG150.0022228769.997350
CGGTACT150.0022228769.997338
CTAGACG207.916045E-569.9972923
CGACTTA207.916045E-569.9972948
AGGGGGG76200.065.0368670
GACGCGA2300.063.91057226
ATACGAG501.4551915E-1162.9975612
CGTATGA1700.061.76231841
ACGCGAA401.0622898E-861.2476327
CGCGAAA401.0622898E-861.2476328
GCGATAA2300.060.8672129
ACGCGAT2200.060.4522127
GCCGACT1050.059.9976846
CTCGAAA352.965735E-759.9976839
CGCGATA2350.059.57216328
AAACTCG1300.059.22847736
ACGAGTT950.058.94508727
TGACGTC1500.058.3310845
ACGACAC308.359802E-658.3310822
Produced by FastQC (version 0.11.5)