FastQCFastQC Report
Wed 25 May 2016
SRR1781065_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781065_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences252581
Sequences flagged as poor quality0
Sequence length76
%GC68

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG9867339.065883815488895No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACAGG150.002222963669.9994054
CGACGAG207.916995E-569.99940522
TGGACTA207.916995E-569.99940522
GCCGCGA150.002222963669.99940524
CTACGGG252.8347185E-669.99940538
CGGGCTA207.916995E-569.99940541
CGCAAAC150.002222963669.99940525
CCGCAAA150.002222963669.99940524
CCAACGT308.361671E-658.3328422
TATGCAC252.3875543E-455.9995233
TAACAGT252.3875543E-455.99952327
CGCGGCT200.006946021752.4995547
TCAGCAT200.006946021752.49955410
TCGCTGA200.006946021752.49955458
GTGTACG200.006946021752.49955430
CGTACAG200.006946021752.4995543
GTGAGCG200.006946021752.49955431
GACGCCA200.006946021752.49955463
TTCGCTG200.006946021752.49955457
CGATATA200.006946021752.49955436
Produced by FastQC (version 0.11.5)