FastQCFastQC Report
Wed 25 May 2016
SRR1781065_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781065_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences252581
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCCAAATAAATCTCGTATGCCGT6347825.131739917095903No Hit
TCTTATACACATCTCCGAGCCCACGAGACCCAAATAAATCTCGTATGCCG76993.0481310945795608No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCCAAATAAATCTCGTATGCC36021.4260771792019193No Hit
CATATACACATCTCCGAGCCCACGAGACCCAAATAAATCTCGTATGCCGT22040.8725913667298807No Hit
ATACACATCTCCGAGCCCACGAGACCCAAATAAATCTCGTATGCCGTCTT17000.6730514171691457No Hit
TCTTTATACACATCTCCGAGCCCACGAGACCCAAATAAATCTCGTATGCC15780.6247500801723012No Hit
CTATACACATCTCCGAGCCCACGAGACCCAAATAAATCTCGTATGCCGTC12630.5000376116968418No Hit
CTTATACACATCTCCGAGCCCACGAGACACAAATAAATCTCGTATGCCGT9790.387598433769761No Hit
CTTTATACACATCTCCGAGCCCACGAGACCCAAATAAATCTCGTATGCCG5820.23042113223084873No Hit
CTTTACACATCTCCGAGCCCACGAGACCCAAATAAATCTCGTATGCCGTC5620.22250288026415288No Hit
ACTTATACACATCTCCGAGCCCACGAGACCCAAATAAATCTCGTATGCCG4760.1884543968073608No Hit
TTATACACATCTCCGAGCCCACGAGACCCAAATAAATCTCGTATGCCGTC4430.17538928106231266No Hit
TCTTAATACACATCTCCGAGCCCACGAGACCCAAATAAATCTCGTATGCC4330.17143015507896478No Hit
CTTATACACATCTCCGAGCCAACGAGACCCAAATAAATCTCGTATGCCGT4190.16588737870227768No Hit
CTTAATACACATCTCCGAGCCCACGAGACCCAAATAAATCTCGTATGCCG3960.1567813889405775No Hit
CTTATACACATCTCCGAGCCCACGAGACCCAAATAATCTCGTATGCCGTC3810.1508426999655556No Hit
TATACACATCTCCGAGCCCACGAGACCCAAATAAATCTCGTATGCCGTCT3420.13540210863049873No Hit
CTAATACACATCTCCGAGCCCACGAGACCCAAATAAATCTCGTATGCCGT3340.13223480784382038No Hit
CTTATACACATCTCCGAGACCACGAGACCCAAATAAATCTCGTATGCCGT3110.12312881808212019No Hit
CTTATACACATCTCCGAGCCCACGAGACCAAAATAAATCTCGTATGCCGT2850.11283509052541561No Hit
CTTATACACATCTCCGAGCCCACGAGACCCAAATAAATCTCGTATGCAGT2640.10452092596038498No Hit
CTTATACACATCTCCGAGCCCACGAGACCCAAATAAATCTCGTATGACGT2560.10135362517370665No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACAGTGG150.002211784770.0884256
CCTCGCG150.002222240270.005159
ATAACCG150.002222240270.0051510
TTCAGGA150.002223986269.99129536
CCGTCAA150.002223986269.99129547
AGACGCG150.002223986269.99129525
CCCCGTT150.002223986269.99129525
TAACCGA150.002223986269.99129511
AACGATA150.002223986269.99129521
GTTCCAG150.002223986269.99129529
GACGCGT150.002223986269.99129526
TCCCCGT150.002223986269.99129524
AAGATCT150.002223986269.99129534
CTCTCGA150.002223986269.99129537
TGTCGGA150.002223986269.99129535
GTATTCT500.069.9912949
TGACGAC401.3460522E-1069.9912945
CCCGTTC207.9215446E-569.9912926
ATCCAGC207.9215446E-569.9912934
ATGACGA500.069.9912944
Produced by FastQC (version 0.11.5)