FastQCFastQC Report
Wed 25 May 2016
SRR1781064_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781064_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences125834
Sequences flagged as poor quality0
Sequence length76
%GC90

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG9305773.95219098176963No Hit
TGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1320.10490010648950204No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGGGCG150.002220388769.999212
TGGCGTC150.002220388769.9992120
TCTCTCA150.002220388769.9992122
ACTGCCA200.00693803852.49940518
GCGTCTG200.00693803852.4994058
TTGTGCG200.00693803852.4994052
CCGATGA200.00693803852.4994059
CGTCTGG200.00693803852.4994059
CGGGCGA305.8596773E-446.66613810
GCGCTGG305.8596773E-446.66613815
GGCGCTG400.002411414634.99960314
CTGGCGC552.9786298E-431.81782212
TCGGTGG450.004294270631.11075645
GCGGGTG703.4667162E-529.999668
TGTTAAA853.9226143E-628.82320270
ATGTTAA1151.4803518E-624.3475569
CGGGTGG800.002654268621.8747529
GGGCGCG1154.082496E-521.3041064
GATGTTA1558.6237196E-720.3223568
GGGGCGC1150.001001316918.2606623
Produced by FastQC (version 0.11.5)