FastQCFastQC Report
Wed 25 May 2016
SRR1781064_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781064_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences125834
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGCCGT5901646.89988397412464No Hit
TCTTATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGCCG73625.8505650301190455No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGCC35992.860117297391802No Hit
CATATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGCCGT16191.2866157000492713No Hit
TCTTTATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGCC15471.2293974601459066No Hit
ATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGCCGTCTT14821.177742104677591TruSeq Adapter, Index 7 (95% over 21bp)
CTATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGCCGTC9770.7764197275776022No Hit
CTTATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTAGTATGCCGT6700.5324475102118664No Hit
CTTATACACATCTCCGAGCCCACGAGACCTTGGGTCATCACGTATGCCGT4700.3735079549247421No Hit
CTTTATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGCCG4420.35125641718454476No Hit
CTTATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGACGT4280.340130648314446No Hit
CTTTACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGCCGTC4020.31946850612711986No Hit
ACTTATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGCCG3750.2980116661633581No Hit
TTATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGCCGTC3530.2805283150817744No Hit
CTTATACACATCTCCGAGCCAACGAGACCTTGGGTCATCTCGTATGCCGT3290.2614555684473195No Hit
TCTTAATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGCC3260.25907147511801265No Hit
CTTATACACATCTCCGAGCCCACGAGACCTAGGGTCATCTCGTATGCCGT3190.2535085906829633No Hit
CTTATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTAAGCCGT3040.24158812403642896No Hit
CTTATACACATCTCCGAGCCCACGAGACATTGGGTCATCTCGTATGCCGT2900.23046235516633023No Hit
TATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGCCGTCT2840.22569416850771654No Hit
CTTAATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGCCG2810.22331007517840964No Hit
CCTTATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGCCG2750.21854188851979595No Hit
CTTATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGCAGT2300.18278048858019297No Hit
CTTATACACATCTCCGAGACCACGAGACCTTGGGTCATCTCGTATGCCGT2040.1621183463928668No Hit
CTTATACACATCTCAGAGCCCACGAGACCTTGGGTCATCTCGTATGCCGT2010.15973425306355993No Hit
CTAATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGCCGT1810.1438402975348475No Hit
TCTATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGCCGT1710.1358933197704913No Hit
CTTATACACATCTCCGAGCCCACGAGACCTTGGGTCATATCGTATGCCGT1500.11920466646534324No Hit
CTTATACACATCTCCGAGCACACGAGACCTTGGGTCATCTCGTATGCCGT1440.1144364798067295No Hit
CTCTATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGCCG1410.11205238647742265No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTAA252.826302E-670.0000148
TCCGTCT252.826302E-670.0000146
ACGTAAG252.826302E-670.0000140
TCAGTCT252.826302E-670.0000146
AGCACAA150.00222028970.017
GAAATTG207.901245E-570.026
ATGGGTA150.00222028970.030
CCTTATA401.3278623E-1070.01
TGATAGA150.00222028970.055
CAAACGA207.901245E-570.019
CGAACGC150.00222028970.030
CGTATGG150.00222028970.043
TATGGCG150.00222028970.045
TGGCGTC150.00222028970.047
GCAAACG207.901245E-570.018
ATTAAGC207.901245E-570.051
AGTGGGG207.901245E-570.070
CCGTCAA301.01688784E-770.047
ATCGTAA207.901245E-570.039
AAGCCGA207.901245E-570.044
Produced by FastQC (version 0.11.5)