FastQCFastQC Report
Wed 25 May 2016
SRR1781063_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781063_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences262400
Sequences flagged as poor quality0
Sequence length76
%GC69

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG10447539.81516768292683No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATACGCG150.002223081769.9992454
TGCTATC207.917666E-569.999242
TGTGCGC352.967281E-759.9993483
GTTGTGC308.324971E-658.3771931
ATCGCGT252.3877551E-455.999396
TCGCGTG252.3877551E-455.999397
CGCGTCT452.6930138E-854.4438557
TAAAGCA200.006946389652.49942837
TGCGGGT200.006946389652.4994285
TGCGCGT407.462586E-752.4994285
CGAGGTT200.006946389652.49942822
TTGCCGG200.006946389652.49942818
CACGTCT200.006946389652.4994287
CTCTAAC200.006946389652.49942831
ATGCTAT305.849348E-446.7017561
CGTGCAC305.87138E-446.6661610
GCGTGCA305.87138E-446.666169
GACGAAA305.87138E-446.6661625
TATCGCG305.87138E-446.666165
GTGCGCG451.6809117E-646.666164
Produced by FastQC (version 0.11.5)